TITLE:
The Two-Component Sensor Protein CovS Affects Penicilling Susceptibility by Modulation of Cell-Wall Synthesis in Streptococcus pyogenes
AUTHORS:
Masaaki Minami, Syun Syun Torii, Michio Ohta
KEYWORDS:
Streptococcus Pyogenes, CovS, pbp2a, glmM
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.3 No.3,
March
18,
2015
ABSTRACT:
In Streptococcus pyogenes, we have
described the two-component signal transduction sensor and regulatory systems,
CovR/S affect the antimicrobial susceptibility including penicillin G before.
But the mechanism how two-component sensor protein CovS modulates penicillin G
susceptibility has not been elucidated till date. This study aimed to determine
how the CovS affected penicillin G susceptibility in Streptococcus pyogenes by
northern blot analysis. At first, we investigated the covS mRNA expression
under penicillin G induction. We found that the decrease of covS mRNA expression
under Penicillin G stimulation. Next we investigated the expression of cell
wall synthesis gene, pbp2a and glmM with use of covS knockout mutants from emm1
Streptococcus pyogenes strain 1529. We found that the cell-wall synthesis gene
expression of the ?covS mutant strain were lower than that of the wild-type
strain. Furthermore the expression of glmM mRNA gene was lower than the
expression of pbp2a mRNA gene in the ?covS mutant strain. The covS-complemented
strain almost restored the mRNA expression compared to covS mutant strain. The
two-component sensor protein CovS affects the susceptibility to penicillin G
in Streptococcus pyogenes by modulation of cell-wall synthesis.