TITLE:
Quantitative Analysis of Matrine and Oxymatrine in Sophora flavescens Extract and Its Biopesticides by UPLC
AUTHORS:
Sung Jin Lim, Du Yun Jeong, Geun Hyoung Choi, Byung Jun Park, Jin Hyo Kim
KEYWORDS:
Matrine, Oxymatrine, Biopesticide, Quantitative Analysis
JOURNAL NAME:
Journal of Agricultural Chemistry and Environment,
Vol.3 No.2,
May
20,
2014
ABSTRACT:
Successive cartridge clean-up method for the
simultaneous determination of matrine and oxyma- trine in
biopesticides containing Sophora
flavescens extract was developed and validated by UPLC. The clean-up method
was established with ENVI-Carb (0.5 g) and C18 SPE (0.5 g)
cartridges for the bioactive alkaloid in biopesticides from S. flavescens, and the eluate was
analyzed to quantify the matrine and oxymatrine by UPLC. The developed method
was validated, and the recovery and LOQ of both materials were 105.0% and 103.6%, and 0.050 and
0.684 mg·kg-1,
respectively. Of the twenty
one samples, the total content
of matrines were analyzed by using the
developed method and the result showed the developed successive clean-up method could contribute to the
manufacture and control of
biopesticides including matrines, and can be ap- plied to the
method development for the analysis of alkaloid materials in biopesticides.