TITLE:
Antimicrobial Resistance in Enterococcus sp Isolated from Soft Cheese in Southern Brazil
AUTHORS:
Luciana Furlaneto-Maia, Kátia Real Rocha, Fernanda Carla Henrique, Amanda Giazzi, Márcia Cristina Furlaneto
KEYWORDS:
Enterococci; Dairy Products; Foods; Antimicrobial Resistance
JOURNAL NAME:
Advances in Microbiology,
Vol.4 No.3,
February
19,
2014
ABSTRACT:
The aim of this research was to study the incidence of
antibiotic resistance in 56 Enterococcus strains isolated from dairy products. The identification of enterococci was detected by polymerase chain reaction (PCR) using specific
primers to E. faecalis, E. faecium, E. gallinarum and E.
casseliflavus, and antibiotic
resistance was tested by the disk diffusion
method. The most prevalent species was E.
faecium with a rate of 58.33%, followed by 27.77% E. faecalis, 11.11% E. casseliflavus and 2.7% E.
gallinarum. Distribution of resistance was found in different
species. All isolates were susceptible to chloramphenicol, ampicillin, imipenem
and amoxicillin/clavulanic acid. In addition, isolates resistant to
tetracyclin, nalidixic acid, amikacin, erythromycin, vancomycin and cephalothin
were detected. A total of 66.6% of E.
faecium and 58.3% of E. faecalis strain were resistant to multiple drugs. The van(A) gene was detected in 100% of vancomycin
resistant enterococci. Considering the results of our study, dairy enterococci
can be considered a potential source for dissemination of antibiotic
resistances.