Synthesis and Physicochemical Characterization of Biodegradable Star-Shaped Poly Lactide-Co-Glycolide-β-Cyclodextrin Copolymer Nanoparticles Containing Albumin ()
The aim of present work was to assess the merits of PLGA-β-CD nanoparticles as an albumin carrier. For this purpose, the copolymers of PLGA-β-CD were synthesized and characterized. Polymeric nanoparticles were prepared by using a precipitation-solvent evaporation technique. To study the effect of experimental parameters on the properties of these nanoparticls, different formulations of monomers were obtained and fully characterized for morphology, size and encapsulation efficiency.
2. Materials and Methods
2.1. Materials
DL-Lactide (LA), glycolide (GA), β-cyclodextrin (β- CD), and stannous octoate were obtained from Sigma Chemical Co. (St. Louis, MO). Dichloromethane (DCM), Polyvinyl alcohol (PVA, Mw 10,000) and solvents were purchased from Merk company. The structure of copolymers was characterized by FT-IR (Shimadzu 8400, Kyoto, Japan), 13CNMR and 1H-NMR ((Bruker spectra spin 400 MHz, Leipzig, Germany, and their molecular weight and molecular weight distribution (polydispersity index, Mw/Mn) was determined by gel permeation chromatography (GPC, Waters model 515 HPLC pump, Milford, USA). Differential scanning calorimetry (DSC) was conducted in nitrogen atmosphere using a (DSC7 Perkin Elmer, Waltham, USA) calorimeter. Samples were sealed in aluminum pans and heated twice in a nitrogen atmosphere. Drug-loaded polymeric particles were lyophilized by a Christ Alpha 1 - 4 freeze dryer.
2.2. Synthesis of β-CD-PLGA Copolymer
β-CD-PLGA Copolymer nanoparticles of five different ratio (according to table 1) were synthesized Bulk polymerization. LA, GA, and β-CD were heated while stirring to 40˚C, in a nitrogen atmosphere followed by the addition of 0.05% of stannous octoate. The temperature was raised to 150˚C. After 15 min the viscous mixture reacted further at 150˚C for 10 h. The mixture was dissolved in dichloromethane (DCM) as much as possible by boiling and the solvent was separated. The solution was treated with aqueous HCl solution to remove the catalyst. The solution was washed with water and diluted with DMC and added drop wise within half an hour to methanol at –30˚C. At this temperature the mixture was stirred and the product was filtered off and lyophilized. The copolymers are referred to in the text as PLGA-β- CDx, where x designates the mole % of β-CD in the copolymer.
2.3. Preparation of Albumin-Loaded Nanoparticles
Drug-loaded nanoparticles PLGA-β-CD nanoparticles were prepared by using a double emulsion method— evaporation (W/O/W). The albumin was dissolved in HCl (0.1 N) and added to polymer solution (with 10 wt%) in Dichloromethane followed by homogenization in a 20,000 rpm and 60 second primary emulsion W/O was established. These emulsions added to aqueous solution of polyvinyl alcohol (PVA) and for a second time were under Homogenizer. During homogenization the temperature was fixed below 10˚C. After the formation of the emulsion W/O/W solvent was evaporated under vacuum. Nanoparticles obtained by centrifugation at 12,000 rpm for 20 min and were isolated. Nanoparticles supernatants were removed and stored for subsequent experiments.
Table 1. Physical properties of star-shaped PLGA–b-CD copolymers.
2.4. Characterization of β-CD-PLGA Copolymer
2.4.1. Fourier Transforms Infrared Spectroscopy
FT-IR spectra obtained by FT-IR spectrophotometer (Shimadzu 8400, Kyoto Japan) for blank and drug loaded nanoparticles using KBr discs.
13CNMR and 1H-NMR spectroscopy The chemical structures of the copolymers were determined by 13 CNMR 1H-NMR (Bruker spectra spin 400 MHz, Leipzig, Germany).
2.4.2. Study of Thermal Properties
Glass transition temperature (Tg) was determined using differential scanning calorimetric measurements (DSC7 Perkin Elemer, Waltham, USA). All measurements were conducted in crimped nonhermetioc aluminium pans by heating the samples at a rate of 10˚C/min from 20 to 200˚C, with an empty crimped aluminum pan used as reference. Tg was considered at the mid-point temperature of the endothermic drift in the heating curve. All DSC tests were carried out under a 2 ml/min flow of nitrogen to prevent oxidation.
2.4.3. Determine of Average Molecular Weight and Polydispersity Index
The molecular weight and molecular weight distribution (polydispersity index, Mw/Mn) of copolymers were determined by gel permeation chromatography (GPC, waters 515 HPLC pump, USA), that is equipped with a refractive index detector, using a series of high-resolution columns (waters, styragel HR4E 7.8 mm × 300 mm and styragel HR3E 7.8 × 300 mm) and tetrahydrofuran (THF) as a mobile phase. Tetrahydrofuran was used as eluent at 45˚C and polystyrene standards (polymer laboratories, Inc. USA). Total time for analyzing of samples which were injected after dissolving in THF solvent was 10 - 15 minutes.
2.4.4. Determination of Particle Size and Morphology of the Drug-Loaded Nanoparticle
The morphology of the polymeric nanoparticles was observed using a scanning electron microscopy (SEM). For the scanning electron microscopy (SEM), the lyophilized nanoparticles were placed on a double stick tape over aluminum stubs to get a uniform layer of particles. Samples were then gold-coated using a sputter gold coater. Gold coated particle samples were cooled over liquid nitrogen prior to SEM observations to avoid their melting under high magnification due to the electron beam exposure (Leo Electron Microscopy Ltd., Cambridge, UK). Prior to examination, samples were prepared on aluminum stubs and coated with gold under argon atmosphere by means of a sputter coater.
2.4.5. Determination of the Albumin Entrapment Efficiency
Loaded drug quantity was determined according to the following method after nanoparticles were formed, unbound albumin was separated by centrifugation for 10 min, and the precipitate nanoparticles were separated. The separated nanoparticles was then lyophilized and resulting powder containing the albumin -loaded nanoparticles was dissolved in DMC to obtain a clear solution and analyzed UV spectrophotometrically (Shimadzu UV- 160). Loading capacity was expressed in terms of entrapped drug content, and entrapment efficiency (EE %) as follows:
3. Result and Discussion
3.1. Synthesis and Characterization of Star-Shaped PLGA-β-CD Copolymer
Star-shaped polymers because of only one branched center are the simplest branched polymers. These polymers can be produced by chemically linking preformed polymers (polymer first method) or by growing polymer chains from a multifunctional initiator (core first method). Coupling agents with a compressed dendritic construction have been used to enhance the functionality of the star polymers. Branched polyesters consisting of PVA grafted with chains of PLGA is typical polyester used for pharmaceutical use. In the present study, β-CD was proffered to previously use multifunctional initiators as the potential of CDs and their derivatives as new biodegradable carrier for peptides, proteins, and oligonucleotides with emphasize on the interaction between CDs, these drug candidates, and various endogenous molecules. Chemical structure of star shaped PLGA-β-CD copolymer is shown in Figure 1. The copolymer was synthesized by bulk polymerization of the lactones (LA and GA) in the presence of the β-CD using sn-octoate as catalyst. The primary hydroxyl groups of β-CD are expected to begin the polymerization of LA and GA, while it is rather debatable whether the secondary OH will act alike in view of its stereochemical inhibition. The yield of polymer synthesis after precipitation with cold ethers given in Table 1. Per mole of β-CD and sn-octoate in all polymerization reactions was 0.1 and 0.05 mole and the variable molar ratios of LA and GA were used. Relatively low amount of β-CD and catalyst was used in order to obtain high molecular weight polymers. Table 1 and GPC choromarograms in figure 2 shows that increasing the molar ratio of LA to GA, yield of polymerization and the molecular weight of the final polymer increase and the best results in molar ratio of 3 to 1(I) is obtained. This shows the higher activity of LA in copolymerization. Another reactions with variable molar of cyclodextrin were prepared and observed that increasing molar ratio of cyclodextrin growing polymers very minor and the molecular weight of the polymers were low, it related to complexation of monomers in the internal cavity of the cyclodextrin and prevents the initiation of the polymerization reaction. Also, decreasing of cyclodextrin molar ratio from 0.1 mole lead to the formation of linear chains of PLGA. Therefore, the appropriate concentration of cyclodextrin was considered 0.1 moles.
3.2. DSC Measurements
DSC is usually used for study of physical state of nanoparticles. DSC was used to determine the thermal properties of star-shaped copolymers. The expected decrease in the glass transition temperatures Tg and melting points Tm could be observed (Table 1). The reduction of interactions between the star-shaped polyester molecules due to their branched structure lowered Tg and Tm compared to linear PLGA copolymers. As shown in Table 1 increasing lactide in a certain concentration melting point of copolymer were increased becouse lactide increasing number of methyl groups and the amorphous nature of the polymer is more.
3.3. NMR and FT-IR Spectra of Copolymer
The chemical composition of the copolymers was determined with H-NMR and C-NMR by integrating the signals pertaining to each monomer. The freeze dried particles dissolved in DMSO showed the same spectra as that of the original copolymer. This indicated that the composition for the particles was exactly the same as that of the original matrix. Although the 1H NMR spectra (figure 3) of star-branched polyesters show the resonance signals for typical linear PLGA protons (the mul-
Figure 1. Chemical structure of PLGA-β-CD star-shaped copolymer.
(a)
(b)
Figure 2. GPC choromarograms of PLGA-β-CD copolymer, a(I) and b (IV).
tiplets at 5.2 and 4.7 ppm related to the LA CH and the GA CH2 respectively, and the singlet at 2.2 ppm correspond to CH3 of LA), there are some other peaks, due to the incorporation of β-CD. The C2 - C6 methine and methylene protons of β-CD appeared at 4 - 4.5 ppm the assignment of the peak at 4 ppm to the methylene groups of β-CD was supported by its increase in resonance intensity with an increase in the β-CD molar ratio. The presence of secondary –OH groups at the chain end and/or unreacted –OH groups of polyol could be appear at 4 and 4.3 ppm which may overlap with the peak of methane protons. LA terminal –OH appears at 2 ppm. The signals of the PLGA-β-CD in the 13C NMR spectra (figure 4) were 21.07 ppm LA CH3, 61 ppm LA CH, 173 ppm esteric CO (polylactide-GA ester) 170.07 ppm methylene CH and 17.03 ppm (CH3). The connecting
Figure 3. 1H NMR spectrum of PLGA-β-CD copolymer.
Figure 4. 13C NMR spectrum of PLGA-β-CD copolymer.
ester bond appeared at about 169 ppm CD-LA ester, and β-CD-CH2-OCO at about 61.2. The FT-IR spectra of these copolymers showed a broad absorption at 2990 cm−1 indicating the presence of –OH groups. In addition, an absorption band at 1760 cm−1 confirms the formation of –O–C–O ester bonds (figure 5). The GPC traces of the star-shaped PLGA-β-CD copolymers give monodisperse curves. In fact, this is in agreement with previous publications where sn-octoate was used for LA-glycerol or LA pentaerythrol. Therefore, it could be suggested that nitiation of polymerization occurs via the polyol
Figure 5. FT-IR spectrum of PLGA-β-CD copolymer.
(β-CD).
3.4. Characterization of Made of PLGA-β-CD Copolymer Nanoparticles
star-shaped PLGA-β-CD(I) copolymer nanoparticles were prepared by a double emulsion-solvent evaporation method in the presence PVA. Figures 6 and 7 shows the SEM and TEM micrographs of the star-shaped PLGA-β- CD nanoparticles. SEM and TEM micrographs of all nanoparticles revealed relatively spherical morphology. The average particle size of samples was 80 - 210 nm.
(a)
(b)
Figure 6. Scanning electron microscopy (SEM) of nanooparticles surface fabricated by PLGA-β-CD(I) without albumin (a), and PLGA-β-CD(I) with albumin (b).
3.5. Effect of Temperature on Yield of Polymerization and Molecular Weight
Polymerizations were conducted in solvent-free and melt conditions. Polymerization performed in 130˚C and 150˚C and 180˚C. Polymerization yield and the molecular weight of the polymers for sample (I) is summarized in Table 2. As Table 2 increasing the temperature of the polymerization reaction from130˚C to 180˚C the yield of polymerization increased and concentration of unreacted monomers reduced. Also increase in the reaction temperature leads to a decrease in the molecular weight of the polymers. As shown in Table 2, between the different
(a)
(b)
Figure 7. Transmission electron microscopy (TEM) nanoparticles surface fabricated by in 50 mg/ml of PLGA-β-CD copolymer (a), and in 35 mg/ml of PLGA-β-CD copolymer (b).
Table 2. The effect of temperature on the reaction yield and the molecular weight of the final polymer.
temperatures, 150˚C is the best temperature for polymerization reaction.
3.6. Effect of Copolymer Concentration on the Nanoparticles Properties
Concentration of the polymer in the emulsion is an important factor on nanoparticles properties. As shown in tables 3 and 4 polymer molecular weight oil/water ratio and temperature plays an important role in particle size.
As shown in Table 3 increase concentration of polymer microparticle size and efficiency is Mykrvankpsvlasyvn. The reason for this is explained so thick and viscous po-
Table 3. The effect of polymer concentration on the nanoparticles properties.
Table 4. Effect of aqueous phase volume on particle size and EE.
lymer solution cannot be easily broken into smaller droplets. On the other hand, the formation of a solid shell around Mykrvzrat high concentration of polymer in the polymer layer creates a viscous drop Mykrvzrat faster done. External aqueous phase of the emulsion layer to prevent diffusion of albumin. The high concentration of polymer increases efficiency and reduces nanoparticles initial burst release is albumin.
3.7. Effect of Aqueous Phase Volume on Particle Size and EE
According to table 4 increasing in the aqueous phase volume led to a decrease of nanoparticles average size. Coalescence of droplets could be prevented by a large quantity of internal phase. An increase in the internal aqueous could lead to an increase in EE. The Precipitation of the polymer solution phase was accelerated and the hardening time was shortened with the increase of inner phase.
3.8. Effect of Temperature on the Albumin Loaded PLGA-β-CD Copolymers Properties with
Temperemper of preparing nanoparticles known to be a key factor to influence the size and encapsulation efficiency of nanoparticles. As shown in table 5 increasing the temperature from 10˚C to 28˚C reduce thenanoparticle size becouse the emulsion W/O/W have lower viscosity in high temperatures, so it is easier to break it into smaller droplets.
When the temperature of encapsulation raised to 38˚C particles size increased due to the high evaporation rate of the solvent. In addition Table 5 shows that in 10˚C to 38˚C encapsulation efficiency is higher than other temperatures. Differences in encapsulation efficiency depend
Table 5. Effect of temperature on the albumin loaded PLGA-β-CD copolymers properties.
on various parameters such as condition of reaction, nanoparticle size, initial loading, polymer concentration, polymer molecular weight, polymer composition, and special techniques are particle. Formation of a solid layer of polymer around nanoparticle increase efficiency). Nanoparticles which prepared at 38˚C quickly become solid and this increased efficiency of encapsulation. The efficiency of prepared nanoparticles in temperatures below and above will be the same [27,28].
4. Conclusion
Biodegradable nanoparticles, as drug delivery paradigms, have been extensively used for delivery of wide ranges of small molecules as well as macromolecules such as peptides, proteins and genes [29]. The morphological modification may improve the physicochemical characteristics of the biodegradable polymers. Star-shaped PLGA-β- CD copolymers with different molar ratios of monomers were synthesized and used for the preparation of albumin nanoparticles [30]. In conclusion, the results obtained in the present study showed that star-shaped PLGA-β-CD could be synthesized by ring-opening polymerization of the LA and GA in the presence of β-CD. The albumin loaded nanoparticles could be prepared by double emulsion technique. It was found that the copolymers’ composition influences the mean geometric diameter of the albumin loaded nanoparticles, as well as their loading efficiency. These results showed that PLGA-β-CD nanoparticles could be an effective carrier for anticancer drug delivery. An increase in the internal aqueous phase volume led to a decrease in particles’ average size. The high entrapment efficiency (EE) (about 70%) was obtained for 80 nm particles [31,32].
Competing Interests
The authors declare that they have no competing interests.
Authors’ Contributions
KN conceived of the study and participated in its design and coordination. AA and SD participated in the sequence alignment and drafted the manuscript. All authors read and approved the final manuscript.
Acknowledgements
The authors thank Department of Medical Nanotechnology, Faculty of Advanced Medical Science of Tabriz University, and Department of Science, Ahar Branch, Islamic Azad University, Ahar, Iran for all supports provided.
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