TITLE:
Novel intron 2 polymorphism in the melanophilin gene is in Hardy-Weinberg equilibrium and is not associated with coat color in goats
AUTHORS:
Mufliat A. Adefenwa, Brilliant O. Agaviezor, Sunday O. Peters, Matthew Wheto, Oludotun J. Ekundayo, Moses Okpeku, Bola O. Oboh, Khalid O. Adekoya, Christian O. N. Ikeobi, Marcos De Donato, Bolaji N. Thomas, Ikhide G. Imumorin
KEYWORDS:
Coat Color; Goats; Melanophilin; Nigeria; Intron 2; SNP; PCR-RFLP
JOURNAL NAME:
Open Journal of Genetics,
Vol.3 No.3,
August
28,
2013
ABSTRACT:
Pigmentation plays important
adaptation and physiological efficiency roles in animals. In the sequence of
a 648 bp fragment representing intron 1, exon 2, and part of intron 2 of the
MLPH mammalian pigmentation gene, we identified a novel g.469C> G mutation in intron 2, and
genotyped it in 266 Nigerian goats using PCR-RFLP analysis. The C allele had
frequencies of 0.9625, 0.9804 and 0.97405
in West African Dwarf (WAD),Sahel(SH) and Red Sokoto (RS) breeds, respectively. The G allele was the highest in
WAD (0.0375), followed by RS (0.02595), and then SH (0.0196). Overall low FIS
and FST and high Nm values demonstrate little differentiation within and among
the goat breeds at this intronic locus. This g.469C> G polymorphism in MLPH gene is the first in
any goat breed and also first in Nigerian goats. Our results suggest that this
intronic SNP locus is maintained at Hardy-Weinberg equilibrium (P