TITLE:
Additive Protective Effects of Luteolin and Pyruvate against 6-Hydroxydopamine and 3-Hydroxykynurenine Induced Neurotoxicity in SH-SY5Y Cells
AUTHORS:
Natalia Wszelaki, Matthias F. Melzig
KEYWORDS:
Flavonoids; Oxidative Stress; Pyruvate; ATP; Luteolin; Neurotoxicity
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.4 No.4,
July
1,
2013
ABSTRACT:
Oxidative
stress has been implicated as one of the causes in cell death in many neurodegenerative disorders. Due to antioxidative properties in vitro, the use of flavonoids and other polyphenolic compounds synthesised by plants are considered to be a promising
strategy to prevent Alzheimer’s disease and Parkinsons’s disease. In the
present study, we tested protective effects of some polyphenols and sodium
pyruvate on 6-hydroxydopamine (6-OHDA), salsolinol and 3-hydroxykynurenine
(3-HK) induced neurotoxicity in human neuroblastoma SH-SY5Y cells. We found
that luteolin prevented from 6-OHDA and 3-HK induced cell viability reduction
and that one of the mechanisms involved in the neuroprotective process was the ability to increase the level of cellular ATP.
However, luteolin was ineffective against salsolinol-induced toxicity. Neither
pre-treatment with flavonoids nor
simultaneous addition had any protective effects on 6-OHDA, salsolinol or 3-HK induced neurotoxicity.
Interestingly, both pre-treatment and co-treatment with pyruvate provided
protection against 6-OHDA, salsolinol or 3-HK induced toxicity.
Moreover, luteolin and sodium pyruvate, administered together, acted
additively, so to achieve the same effect, lower concentrations were needed.
The ability of luteolin and sodium pyruvate to reduce toxicity of 6-OHDA and
3-HK in SH-SY5Y cells may be related to two different neuroprotective mechanisms and the capability to penetrate into the
cell.