TITLE:
Effect of Dai-Saiko-To (Da-Chai-Hu-Tang) on LDL-Receptor Gene Expression in Human Hepatoma Cell Line (HepG2)
AUTHORS:
Akira Iizuka, Fumihiko Yoshie, Sakae Amagaya, Takaaki Yasuda, Maki Iizuka, Haruyo Yamaguchi, Seiji Nagumo, Kazuo Kondo
KEYWORDS:
Kampo; Dai-Saiko-To; LDL Receptor; Gene Expression; HepG2
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.2A,
February
28,
2013
ABSTRACT:
We
previously reported that Dai-saiko-to (Da-Chai-Hu-Tang), a traditional Japanese
kampo medicine, increased LDL receptor mRNA expression in the liver of the
hypercholesterolemic rabbits. In this study, we focused on LDL receptor gene
expression in a human hepatoma cell line (HepG2) treated with Dai-saiko-to
extract and the extracts of eight herbs presented in Dai-saiko-to. Dai-saiko-to
extract significantly increased LDL receptor gene and SREBP2 gene expression
compared with the control. The extracts of four herbs, Bupleurum root, Pinellia
tuber, Scutellaria root and Peony root significantly increased the LDL receptor
gene expression. Whereas, Jujube, Immature orange, Ginger and Rhubarb extracts
did not change the gene expression. These
results suggest that Dai-saiko-to increased the expression of the cholesterol
transport gene (LDL receptor) regulated by SREBP2 gene
in the human hepatoma
cell line. The pharmacological activity of Dai-saiko-to against
hypercholesterolemia and atheromatous lesions related for these four herbal
components.