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http://dx.doi.org/10.1021/ja01072a017

has been cited by the following article:

• TITLE: Heat-Resistant Properties of α-Chymotrypsin Adsorbed onto Biomass Charcoal Powder

  AUTHORS: Hidetaka Noritomi, Shunichi Kurihara, Nobuyuki Endo, Satoru Kato

  KEYWORDS: Adsorption, Biomass Charcoal Powder, α-Chymotrypsin, Activity, Thermal Stability

  JOURNAL NAME: Journal of Biomaterials and Nanobiotechnology, Vol.5 No.3, July 21, 2014

  ABSTRACT: Biomass charcoal powder (BCP) was used as a carrier for immobilization of α-chymotrypsin through adsorption. BCP was derived from plant biomass wastes such as dumped bamboos by oxygen-free pyrolysis at low temperatures and grinding with a jet mill. The activity of adsorbed α-chymotrypsin was strongly dependent upon the kind of BCP. The thermal denaturation curve of adsorbed α-chymotrypsin was shifted to high temperature, compared to that of free one. When α-chymotrypsin adsorbed onto BCP of bamboos was incubated at 45°C, the half-life of adsorbed α-chymotrypsin was 2.6 times greater than that of free one. After incubation at 45°C, the remaining activity of adsorbed α-chymotrypsin markedly depended on the kind of BCP.