Noritomi, H., Hishinuma, K., Kurihara, S., Nishigami, J., Takemoto, T., Endo, N. and Kato, S. (2013) Adsorption of α-Chymotrypsin on Plant Biomass Charcoal. Journal of Surface Engineered Materials and Advanced Technology, 3, 269-274.
http://dx.doi.org/10.4236/jsemat.2013.34036

has been cited by the following article:

• TITLE: Heat-Resistant Properties of α-Chymotrypsin Adsorbed onto Biomass Charcoal Powder

  AUTHORS: Hidetaka Noritomi, Shunichi Kurihara, Nobuyuki Endo, Satoru Kato

  KEYWORDS: Adsorption, Biomass Charcoal Powder, α-Chymotrypsin, Activity, Thermal Stability

  JOURNAL NAME: Journal of Biomaterials and Nanobiotechnology, Vol.5 No.3, July 21, 2014

  ABSTRACT: Biomass charcoal powder (BCP) was used as a carrier for immobilization of α-chymotrypsin through adsorption. BCP was derived from plant biomass wastes such as dumped bamboos by oxygen-free pyrolysis at low temperatures and grinding with a jet mill. The activity of adsorbed α-chymotrypsin was strongly dependent upon the kind of BCP. The thermal denaturation curve of adsorbed α-chymotrypsin was shifted to high temperature, compared to that of free one. When α-chymotrypsin adsorbed onto BCP of bamboos was incubated at 45°C, the half-life of adsorbed α-chymotrypsin was 2.6 times greater than that of free one. After incubation at 45°C, the remaining activity of adsorbed α-chymotrypsin markedly depended on the kind of BCP.