A. T. Lisa, P. R. Beassoni, M. J. Massimelli, L. H. Otero and C. E. Domenech, “A Glance on Pseudomonas aeruginosa Phosphorylcholine Phosphatase, an Enzyme Whose Synthesis Depends on the Presence of Choline in Its Environment,” In: A. Méndez-Vilas, Ed., Communicating Current Research and Educational Topics and Trends in Applied Microbiology, Badajoz, Vol. 1, 2007, pp. 255-262.

has been cited by the following article:

• TITLE: Choline Promotes Growth and Tabtoxin Production in a Pseudomonas syringae Strain

  AUTHORS: Lucas A. Gallarato, Emiliano D. Primo, Ángela T. Lisa, Mónica N. Garrido

  KEYWORDS: Choline; Pseudomonas syringae; Phytotoxins; Phosphorylcholine Phosphatase

  JOURNAL NAME: Advances in Microbiology, Vol.2 No.3, September 25, 2012

  ABSTRACT: Some Pseudomonas syringae pathovars secrete tabtoxin, a monocyclic β-lactam antibiotic, responsible for chlorosis, the principal halo blight symptom in susceptible plants as oats, rye, barley, wheat and sorghum, among other. Here, we demonstrated that the production of tabtoxin in a P. syringae strain increased at least 150%, when choline, betaine or dimethylglycine were used as nitrogen source, or when choline was added as osmoprotectant in hyperosmolar culture media. Besides, we investigated the induction of phosphorylcholine phosphatase (PchP) activity when choline or its metabolites were used as nitrogen sources. PchP is an enzyme involved in Pseudomonas aeruginosa pathogenesis through its contribution to the breakdown of choline-containing compounds of the host cells. Considering these results and that the success of a pathogenic microorganism depends on its ability to survive and proliferate in its target tissue, we propose that choline is one of the plant signals that contribute to establishment of the infection by tabtoxin-producing strains of P. syringae.