TITLE:
C5L2 Deficiency Enhances Development of Atherosclerosis in ApoE Knockout Mice
AUTHORS:
Yan Liu, Alexandre Fisette, Marc Lapointe, Katherine Cianflone
KEYWORDS:
Atherosclerosis, C5L2, Inflammation, Metabolism, Lipid, Glucose
JOURNAL NAME:
Chinese Medicine,
Vol.6 No.1,
March
26,
2015
ABSTRACT: Background: The complement system is important in development of atherosclerosis via regulation of lipid and glucose metabolism as well as inflammation. Aim: The aim of the present study was to further analyze the contribution of C5L2 to the development of atherosclerosis. We proposed that, with DIO feeding, C5L2 deficiency would promote a phenotype that encourages atherosclerosis development. Coupled to ApoE deficiency, double knockout (2KO) mice would show exacerbated atherosclerotic plaque formation. Methods: First, Wildtype (WT) and C5L2-/-(C5L2KO) and subsequently, ApoE-/-(ApoEKO) and C5L2/ApoE double knockout mice were placed on diets inducing obesity (DIO) or standard chow diet for 12 - 15 weeks. Plasma lipids, glucose, cytokines and hepatic glycogen and lipid contents, mRNA levels and enzyme activities and atherosclerotic plaque size were measured. Results: C5L2KO had increased hepatic glucose oxidation (+90%, p