L-Arginine Supplementation Alters Maternal Blood Biochemical Attributes and Milk Composition Relative to Neonatal Traits of Najdi Ewes ()
1. Introduction
Najdi ewes represent a valuable sheep population in the Kingdom of Saudi Arabia. However, these animals are bred under hot climates, sandstorms, lack of fresh water and scarcity of green forages resulting in reduced productivity and profitability. Several studies have been focusing on the roles of essential and nonessential amino acids on animal growth, development, reproduction, production, and health wellbeing aspects [1] . L-arginine is considered a nutritionally semi-essential amino acid in case of gestating mammals [2] . It has been found that dietary supplementation or intravenous administration of L-arginine is effective in improving functions of reproduction, cardio vascularity, pulmonary system, renal filtration, gastrointestinal, liver, and immunity, as well as facilitating wound healing, enhancing insulin sensitivity and maintaining tissue integrity [3] . Dietary supplementation of L-arginine increased nitric oxide (NO) synthesis in various tissues within physiological ranges [4] . NO is considered an endothelium-derived relaxing factor essential for increasing systemic vasodilatation [5] [6] . Also, NO is essential to placental growth and angiogenesis (the growth of new vessels from the existing vasculature) and therefore, for increasing uterine and placental-fetal blood flow [7] . Moreover, arginine was found to enhance embryonic implantation and development in rats [8] , sheep [9] and pigs [10] . It has been recently reported that supplementing pregnant ewes with a low dosage of L-arginine at the early stage of gestation increased lamb birth weight and survival, and improved maternal health [11] . However, little studies have been conducted to relate the maternal health and neonatal traits with subsequent milk composition in ewes given L-arginine during gestation. Therefore, the aim of the present study was to investigate the effects of oral administration of L-arginine during two stages of pregnancy on the maternal and neonatal health wellbeing, and subsequent milk composition in Najdi ewes.
2. Materials and Methods
2.1. Animals and Location
This study has been conducted during September 2017 and June 2018. Pregnant Najdi ewes at their first to third parity with age of 12 - 30 month and weight of 40 - 60 kg (average 51.4 ± 8.7 kg) were reared in the same breeding farm, tested to be free from mandatory reportable diseases and located in animal production research unit, Agricultural experimental station, Qassim University, Kingdom of Saudi Arabia. Ewes were maintained in semi shaded pens, offered 300 g barley/head/day as a concentrate in addition to alfalfa hay ad lib according to NRC requirements [12] . This diet contains 60% TDN, 2.2 Mcal/Kg ME and 13% crude proteins. Mineral-balanced mixture blocks and clean drinking water were offered as free choice. An approval of the Qassim University animal rights, ethics and use was confirmed.
2.2. Experimental Design
Thirty adult pregnant Najdi ewes were randomly assigned into six groups (n = 5 ewes/group) as follow; first group (CE) was defined as control early pregnancy in which ewes were at their early (first 60 days post breeding) stage of pregnancy and given orally daily 50 ml normal saline (0.9% NaCl), second group (CL) ewes were in late (last 60 days) pregnancy stage and orally administered 50 ml normal saline daily until parturition, third group (LE) ewes were given daily low dose of L-arginine [Herbstore, Diamond Bar, CA USA (75 mg/kg B.W/day)] during the first 60 days of pregnancy, group four (LL) ewes were given daily similar dose of group 3 during their last 60 days of gestation, group five (HE) ewes were given daily high dose of L-arginine (150 mg/kg B.W/day) during the early 60 days of pregnancy and group six (HL) ewes were given similar dose to group five during their last 60 days of pregnancy.
2.3. Blood and Milk Sampling and Analyses
Blood samples for plasma were collected via the jugular vein in heparinized Vacutainer® tubes (Becton Dickinson, Oxford, UK). Sampling just started before the commencement of the treatment (d = 0) and proceeded once per a week until parturition in the late pregnant groups, however in the early pregnant ewes, blood samples were collected once per a week during treatment period and proceeded in a once per month thereafter until parturition. Plasma was separated immediately after blood collection by centrifugation (1500 × g for 15 min/5˚C), harvested, aliquot and stored at −20˚C until analysis. Blood biochemical constituents were determined by commercial reagents (Human Gesellschaft für Biochemica und Diagnostica mbH, Germany). Total protein was determined according to Weichselbaum [13] . Albumin was determined according to Doumas et al. [14] . Globulins were mathematically calculated by the subtraction of albumin value of the total protein value. Cholesterol and Triglycerides were determined according to Seftel et al. [15] . LDL was determined according to Okada et al. [16] . HDL was determined according to Gordon et al. [17] . Creatinine was determined according to Bartels and Boehmer [18] . Urea was determined according to Fawcett and Scott [19] . Aspartate aminotransferase and alanine aminotransferase were determined according to Thefeld et al. [20] .
Milk samples were collected in clean tubes containing potassium dichromate (0.1 ml, 0.3%). Sampling started soon after the end of colostrum and once per a week until weaning at two months of neonate age. Milk samples were kept in the fridge (5˚C) until proximate analysis was performed. Milk samples were analyzed using FUNK GERBER (Lactostar, Germany) milk analyzer according to the AOAC [21] . Briefly, 30 µl of each milk sample was withdrawn and percentage of milk fat, protein, total solids, solids not fat, lactose and ash were determined. A duplicate of each sample were determined to estimate the precision of the assay.
2.4. Offspring Traits
Offspring were weighed at birth and at weaning (day 60) using a scale balance. Also percentage of survival at birth and weaning were recorded within each treatment.
2.5. Statistical Analysis
All variables data were analyzed by the least square analysis of variances for repeated measures procedure of SAS [22] . Mean comparisons among treatments were achieved by the Duncan’s Multiple Range Test [23] . The significance level was considered at P < 0.05.
3. Results
3.1. Lamb Traits
As depicted in Figure 1, ewes given the low arginine during early pregnancy gave birth to the heaviest lambs (4.99 kg) representing about 33% increase in body weight compared to their control counterparts (3.76 kg). Increasing the arginine dose given at early gestation didn’t exert better enhancement on neonatal birth weight (4.99 vs 4.76 for low vs high). However, giving L-arginine at late gestation was in favor of the low dose (4.63 kg) than the high dose (3.76 kg) on birth weight. The ultimate finding is that giving low dose (75 mg/kg B.W) of L-arginine either at early or late gestation was beneficial to the growth of sheep
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Figure 1. Effect of L-arginine dose × stage of pregnancy at which arginine was given on lamb’s birth and weaning weights of Najdi ewes (CE = Saline at early pregnancy, CL = Saline at late pregnancy, LE = 75 mg/kg BW at early pregnancy, LL = 75 mg/kg BW at late pregnancy, HE = 150 mg/kg BW at early pregnancy, HL = 150 mg/kg BW at late pregnancy).
fetus, however giving a high dose (150 mg/kg B.W) at late gestation was not as effective in this respect as when given at early gestation. The heaviest (P < 0.05) lamb weaning weight at 60 days age (Figure 1) was derived from ewes given the low dose of arginine at early pregnancy (18.4 kg) representing about 33% above control (13.84 kg) lamb’s weight. No significant differences (P > 0.05) were found among other treatments (15.63, 15.95 and 16.21 kg in LL, HE and HL, respectively), even though they surpassed the control by 2.38 kg representing + 15% heavier weight.
The highest (P < 0.05) lamb growth rate (Figure 2), based on the duration between birth and weaning (i.e. 60 days), resulted from ewes given the low arginine at early pregnancy (EL; 224 g/head/day), however the lowest (P < 0.05) growth rate was found in lambs born of control ewes (166 g/head/day). Overall, there was no difference (P > 0.05) in the growth rate between lambs born of low (203 g/head/day) or high (197g/head/day) arginine-treated ewes. The best treatment (i.e. EL; the low arginine at early pregnancy) surpassed the control by 33% increase in the daily growth rate (Figure 2).
3.2. Blood Metabolites in Pregnant Ewes
As pregnancy advances, plasma protein (Figure 3) somewhat diminished in control ewes. Clearly, arginine at either level didn’t raise blood plasma protein over control, yet rather it kept up the protein level at late pregnancy to its underlying level. The sole treatment that showed a reduced (P < 0.05) level of albumin was HE (Figure 3). Ewes given high arginine at their early pregnancy have shown the lowest albumin concentration (3.38 g/dl) being 25% less than in control (4.73 g/dL). Whereas, other treatments exhibited a tendency (P > 0.05) of albumin reduction compared with control. There was a critical (P < 0.05) increment in globulins in ewe’s given the low-dose of L-arginine at late pregnancy
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Figure 2. Comparison between growth rates in lambs born of Najdi ewes given low arginine at an early pregnancy relative to the early control lambs.
(LL; 2.28 g/dl), representing +54% above control (Figure 3). However other treatments (i.e. 1.61, 1.50 and 1.74 g/dl in LE, HE and HL, respectively) were not distinctive in globulins than control (1.45 g/dL).
Generally, L-arginine diminished (P < 0.01) blood cholesterol compared with control (Figure 4). Percent of cholesterol reduction ranges between 5% - 24% of the corresponding control. Cholesterol levels were 57.95, 61.12, 47.33, 46.7, 50.4 and 57.83 mg/dl for CE, CL, LE, LL, HE and HL, respectively. Overall, stage of pregnancy didn’t show a difference in blood cholesterol; however the limiting factor in cholesterol was the dose of L-arginine. The low arginine reduced (P <
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Figure 3. Effect of L-arginine dose × stage of pregnancy on plasma total protein, albumin and globulins in Najdi ewes. (CE = Saline at early pregnancy, CL = Saline at late pregnancy, LE = 75 mg/kg BW at early pregnancy, LL = 75 mg/Kg BW at late pregnancy, HE = 150 mg/kg BW at early pregnancy, HL = 150 mg/kg BW at late pregnancy).
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Figure 4. Effect of L-arginine dose × stage of pregnancy on plasma cholesterol, triglycerides, LDL and HDL in Najdi ewes. (CE = Saline at early pregnancy, CL = Saline at late pregnancy, LE = 75 mg/kg BW at early pregnancy, LL = 75 mg/kg BW at late pregnancy, HE = 150 mg/kg BW at early pregnancy, HL = 150 mg/kg BW at late pregnancy).
0.05) blood cholesterol by 21% compared with control figures, however high arginine slightly (P > 0.05) reduced this value by barely 9%. Administration of either dose of L-arginine at early gestation reduced (P < 0.05) triglycerides (TG) by 30% - 41.5% of the control value (Figure 4). Contrariwise, giving L-arginine didn’t change TG than control level, therefore the limiting factor for blood TG was pregnancy stage, not arginine dosage. Giving L-arginine at early gestation reduced (P < 0.05) TG (25.54 mg/dl) by 30% - 41% of their control (43.68 mg/dL) counterparts. On the contrary, giving low L-arginine at late gestation slightly elevated TG (42.6 mg/dL) than its control (38.25 mg/dL) counterpart, while giving high L-arginine at late gestation significantly (P < 0.05) elevated blood TG by 27% compared with its control counterpart. Plasma LDL levels were diminished (P < 0.01) as a result of giving L-arginine. The highest impact in LDL reduction was found in LE ewes (35.23 g/dL) representing 45% below control value (63.55 g/dL). Interaction (P < 0.01) between arginine dosage and stage of pregnancy was attained. The reduction values in LDL were 36%, 28% and 19% in LL, HE and HL compared to control ewes (Figure 4). Plasma HDL profile (Figure 4) exhibited similar levels and trends in treatments as these found in control. The stage of pregnancy exhibited significant effect on plasma HDL level being higher at early (31.83 g/dL) than at late pregnancy (26.53 g/dL).
As shown in Figure 5, giving the high dose of L-arginine at either early (4.72 g/dL) or late (4.07 g/dL) gestation significantly (P < 0.01) increased creatinine levels compared with control (3.64 g/dL). Contrariwise, giving low L-arginine at early pregnancy didn’t change (3.86 g/dL) creatinine level than control or even significantly (P < 0.01) diminished creatinine when given at late pregnancy (2.93 g/dL). Overall, low arginine didn’t change blood creatinine (P > 0.05), while high arginine elevated creatinine by 21% over the control value.
In the ordinary circumstances (i.e. control) urea profile (Figure 6) exhibited a significant (P < 0.01) decline at early (62.07 g/dL) versus late (75.03 g/dL) pregnancy.
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Figure 5. Effect of dosage of L-arginine × stage of pregnancy on plasma creatinine in Najdi ewes. (CE = Saline at early pregnancy, CL = Saline at late pregnancy, LE = 75 mg/kg BW at early pregnancy, LL = 75 mg/kg BW at late pregnancy, HE = 150 mg/kg BW at early pregnancy, HL = 150 mg/kg BW at late pregnancy).
Administration of the high dose of L-arginine increased (P < 0.01) maternal blood urea at either stage by 13% - 19%. Reversibly, giving the low dose (LE &LL) of L-arginine not only maintained urea at its basic levels, but it also normalized the blood urea concentrations throughout pregnancy.
Administration of L-arginine significantly (P < 0.01) increased AST activity (Figure 7). Differences due stage of pregnancy were non-significant (P > 0.05). However, there were significant differences (P < 0.05) due to L-arginine dosage on AST. The increase of AST activity above the control reached 55% in low and 70% in high dose-treated ewes. Concomitantly, ALT activity increased (P < 0.05) in all treatments, though the highest value was found in HL ewes (32.91 U/L) representing 38% above control. Low dosage of L-arginine optimizes the activity
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Figure 6. Effect of dosage of L-arginine × stage of pregnancy on plasma urea in Najdi ewes. (CE = Saline at early pregnancy, CL = Saline at late pregnancy, LE = 75 mg/kg BW at early pregnancy, LL = 75 mg/KG BW at late pregnancy, HE = 150 mg/kg BW at early pregnancy, HL = 150 mg/kg BW at late pregnancy).
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Figure 7. Effect of dosage of L-arginine × stage of pregnancy on plasma AST and ALT in Najdi ewes. (CE = Saline at early pregnancy, CL = Saline at late pregnancy, LE = 75 mg/kg BW at early pregnancy, LL = 75 mg/kg BW at late pregnancy, HE = 150 mg/kg BW at early pregnancy, HL = 150 mg/ kg BW at late pregnancy).
of ALT throughout pregnancy (Figure 7).
3.3. Milk Composition
Table 1 presents data of milk analysis. Ewes given low arginine increased (P < 0.05) total solids (TS), however ewes given high arginine slightly (P > 0.10) decreased milk TS. Likewise, low arginine maintained milk fat percent (8.47%) compared with control (8.29%), however high arginine decreased (P < 0.05) fat percent (7.03%). There were slight (P = 0.06) increases in protein due to treatment. Solids nonfat (SNF) was higher in LE, LL and HL than in CE. Percent of milk lactose was not different in LE, LL and HE than in CL. However, milk ash was higher (P < 0.05) in high than in low-arginine or control ewes.
4. Discussion
The increase in birth weight in the treated ewes might be ascribed to the anabolic effects of L arginine due to its angiogenic effect on the placental blood vessels. The low dosage of arginine given at early gestation resulted in the best birth (4.99 kg) and weaning (18.4 kg) weights. During early days of pregnancy the mothers require protein as a signal for the maternal recognition of pregnancy [11] . Moreover, survival rates at birth and weaning were 100% in the low dose-early pregnancy ewe’s offspring. The high ambient temperature in which these animals were raised represents a challenge at the early implantation. This was alleviated by the supplementation of L-arginine at this early period. The susceptibility of the sheep fetus to the heat stress declines by the progress of pregnancy [24] . Evidently, intrauterine growth restriction (IUGR) has long been known as a common reproductive drawback causing pulmonary atresia in sheep fetuses [25] . However, supplementary L-arginine in the diets of mammals had shown beneficial functions on the fetal growth and development due to its activation of nitrous oxide (NO) and polyamines [26] . The less effectiveness of the high dose of L-arginine on the neonatal birth weights or the survival rates at birth in the current study might be attributed mainly to the edematic effects of
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Table 1. Effect of dose of L-arginine × stage of pregnancy on postpartum milk composition in Najdi ewes.
*a,b,c,dMeans in the same row with different superscripts significantly (P < 0.05) differ. CE = Saline at early pregnancy, CL = Saline at late pregnancy, LE = 75 mg/kg BW at early pregnancy, LL = 75 mg/kg BW at late pregnancy, HE = 150 mg/kg BW at early pregnancy, HL = 150 mg/kg BW at late pregnancy.
such a dose on the maternal tissues and its udder.
The congested teats impeded the natural suckling of the neonates resulting in a high percent of mortality. Overall, the best treatment in milk yield (visual observation, not determined) and composition was found in LE-ewes resulting in the heaviest lamb weaning weights. Arginine serves as a common precursor for the synthesis of NO (a vasodilator and a signaling molecule) and polyamines (key regulators of DNA and protein synthesis) that are crucial for placental angiogenesis and growth in mammals [27] [28] [29] [30] . Moreover, Saevre et al. also found that during the early stages of embryogenesis, the supplemental arginine could have rescued weaker embryos entering the early stages of regression through its role on nitric oxide and polyamine synthesis [31] . Polyamines and nitric oxide may have directly enhanced embryonic cellular proliferation and differentiation to ensure and promote embryonic survival [32] .
The lowering effect of L-arginine on blood lipid fractions, in the current study agrees with a previous study [6] . Likewise, declines in plasma protein and albumin as a consequence to L arginine supplementation were found in pigs [33] . It is previously mentioned that supplementation with L-arginine enhances immunoglobulin’s production [34] . The elevation of creatinine and urea in the high arginine-supplemented ewes would unravel the edema found in these groups which burdened the postnatal maternity and the ability of neonates for normal suckling. In a study on rats, to investigate the effects of acute versus chronic supplementation of L-arginine on renal ischemia, there found deleterious effects due to the chronic, but not acute treatment on the renal function. The chronic supplementation of L-arginine increased blood urea causing partial damage to the nephrons [35] . This finding was supported by Ingram et al. who documented that the reduced early cell proliferation in the glomerulus following two weeks supplementation with L arginine is mediated, in part, by the attenuation of local endothelin-1 (ET-1) production which is further supported by the observation that treatment of uremic animals with a NO donor reduced ET-1 excretion [36] . The main biomarkers of the integrity of the hepatic cells are AST and ALT. In the current study, giving arginine significantly increased AST at all tested levels, however the sole increase in ALT was found in ewes given high arginine at late gestation. The increases of AST and ALT were observed in rabbits given L-arginine [37] . In spite of such increases of the activity of hepatic enzymes, it looks understandable that these tested doses of L-arginine are still within the physiological accepted levels. This suggestion would be reasonable with the fact that both doses of L-arginine at either stage of gestation resulted in higher, or even similar, neonatal birth weight and comparable survival. Milk production is influenced by the angiogenesis of mammary tissue and blood flow to mammary glands, which enhance nutrient delivery to the mammary gland for milk synthesis [38] . Mammary gland blood flow and angiogenesis are regulated by arginine-derived nitric oxide [39] . Furthermore, milk production is highly correlated with mammary gland growth and arginine is required for optimal mammary gland growth [40] . The increase of insulin release in the ewes given arginine would also explain the higher metabolic turnover in mammary cells [11] .
5. Conclusion
The dosage and duration of administration of L-arginine must be considered when designing an experiment to test its effect on cell biology. Moreover, type of animals (i.e. monogastrics vs. ruminants) is of great concern with respect of timing and duration of administration. Drenching pregnant ewes at their early (~first 60 days post breeding) gestation with a daily dose of 75 mg L-arginine per kilogram body weight would be a beneficial approach to obtain heavy and healthy offspring and maintain the maternal health wellbeing. Moreover, this study must be furthered by using larger numbers of animals and also to quantitate the milk yield and its chemical composition and correlate such traits with neonatal survival and growth.
Acknowledgements
The authors acknowledge Qassim University for providing the lodging for animals, and the farm crew for their assistance throughout the experiment.
Compliance with Ethical Standards
This study was approved by the Animal Care and Welfare Committee, Deanship of Scientific Research, Qassim University, Kingdom of Saudi Arabia.