TITLE:
Detecting Concentration of Analytes with DETECHIP: A Molecular Sensing Array
AUTHORS:
Hannah Johnke, Gary Batres, Mark Wilson, Andrea E. Holmes, Sharmin Sikich
KEYWORDS:
Colorimetric Arrays; Sensors; Analyte Concentration; RGB Analysis; Drug Detection
JOURNAL NAME:
Journal of Sensor Technology,
Vol.3 No.3,
September
12,
2013
ABSTRACT: DETECHIP is a detection system made of various sensors that has been shown to detect and discriminate between small molecules of interest, including various illicit and over-the-counter drugs. Previously, detection was normalized to a single concentration of analyte. Now this detection assay can detect concentration differences in analytes via red, green, and blue color value changes and shifts in the UV-Vis spectra of the assay. To determine the concentrations differences, the exposed assays were scanned on a flatbed scanner and the images were analyzed for individual RGB values with a custom macro in ImageJ, an image analysis program. Increasing concentrations of the analyte resulted in greater differences in color values between control and analyte wells. These differences showed a linear relationship to concentration change, some with correlation coefficients greater than 98%. This work expands the capability of DETECHIP to give information about the concentration of analyte when the analyte identity is known.