Abu-Duhier, F., Goodeve, A., Wilson, G., et al. (2000) FLT3 Internal Tandem Duplication Mutations in Adult Acute Myeloid Leukaemia Define a High-Risk Group. British Journal of Haematology, 1, 190-195.
https://doi.org/10.1046/j.1365-2141.2000.02317.x

has been cited by the following article:

• TITLE: Next Generation Sequencing in Oncological Diagnostics: Hype or Hope?

  AUTHORS: Rana Hallak, Manfred Kuepper, Amer Al Chikh Youssef

  KEYWORDS: Oncology, Next Generation Sequencing, Tumor Diagnosis, Personalized Medicine

  JOURNAL NAME: Journal of Biosciences and Medicines, Vol.12 No.2, February 23, 2024

  ABSTRACT: The understanding of how genetic and epigenetic factors influence tumorigenesis, progression and invasion, is vastly growing since new technologies allow the analysis of the functional genome namely the exome, the transcriptome and the epigenome, besides enabling genome-wide assessment of genetic variations. With the advent of new drugs that are indicated tissue agnostic, depending on certain mutations, there is a growing demand for fast and cost-effective genetic diagnosis. The method in focus that already became an indispensable tool in viral diagnosis is next-generation sequencing (NGS). This approach allows sequencing of literally every DNA molecule in the sample and can either be used to assess numerous genetic markers of one patient at a time, or to assess fewer markers of many patients in parallel, which reduces costs. We submitted 23 samples of different tumor entities to four diagnostic companies with different analysis profiles. The results as disclosed and discussed in this report indicate that so far, the main application of NGS is rather in cancer research than in diagnosis, as none of the reports had a real impact on the therapeutic scheme. We are perfectly aware that such a small cohort cannot be generalized, but considering the costs vs. benefits, NGS should be engaged upon a very stringent evaluation only. However, in cases where obtaining a tissue biopsy is impossible or unfavorable, analysis of liquid biopsy by NGS provides a vital alternative.