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has been cited by the following article:

• TITLE: In Vitro Evaluation of Two Tissue Substitutes for Gingival Augmentation

  AUTHORS: Tulio Cardoso dos Santos, Carlos Nelson Elias, Alexandre Barboza Lemos, Andresa Borges Soares, André Antonio Pelegrine, Lucas Novaes Teixeira, Elizabeth Ferreira Martinez

  KEYWORDS: Collagen Matrices, Gingival Recession, Periodontal Regeneration, Matrix Metalloproteinases

  JOURNAL NAME: Journal of Biomaterials and Nanobiotechnology, Vol.14 No.2, April 19, 2023

  ABSTRACT: Three-dimensional collagen matrices of porcine origin are being used as substitutes for soft tissue grafts in periodontal plastic surgery in search of aesthetic and natural results. This in vitro study aimed to compare Fibro-Gide® (GeistlichBiomaterials) and Mucoderm® (BotissBiomaterials) matrices during the initial phase of soft tissue formation. For this purpose, samples of 5 × 5 mm were obtained, and then human fibroblasts were plated on them. After 24, 48 and 72 h, cell viability was assessed using an MTT assay, and the secretion of type I collagen, MMP-2, TIMP-1 and TIMP-2 was analyzed by ELISA immunoassay. The control group (C) consisted of cells plated on polystyrene without the matrices. The morphology of the surfaces was also examined using scanning electron microscopy (SEM), as was the average roughness (Ra) of the samples by a profilometer. Topographic analysis revealed that roughness was significantly higher on Mucoderm® than on Fibro-Gide® (p 0.05). The synthesis of type I collagen, MMP-2 and TIMP-1 were significantly higher from cells plated on Fibro-Gide® than on Mucoderm®, in all time points (p ® than on Mucoderm® (p ® induced an increase in type I collagen, MMP-2 and TIMP-1 and TIMP-2.