TITLE:
Upregulation of GLE1 and LCP2 Genes in H5N1 Influenza Virus Infected Patients
AUTHORS:
Wenfeng Peng, Yaping Shi, Baolin Wang, Bo Liu, Zhiyuan Peng, Jianyong Zhang, Ling Chen, Hong Zhang
KEYWORDS:
Influenza, H5N1, GLE1, LCP2, Upregulation, Host Defense
JOURNAL NAME:
Advances in Infectious Diseases,
Vol.6 No.3,
September
29,
2016
ABSTRACT: Previous study showed that
the Gle1 RNA export mediator-like (Gle1l) gene and the lymphocyte
cytosolic protein 2 (Lcp2) gene were upregulated in response to
influenza virus A/Puerto Rico/8/1934 (H1N1) in a mouse mode. To determine
whether these two genes were upregulated in humans after influenza A virus
infection, nasopharyngeal swabs were collected from eleven patients with
flu-like symptoms for viral RNA extraction and PCR amplification. Sequencing
analysis revealed that nucleoprotein (NP) gene fragments amplified from
nasopharyngeal swabs of four patients shared the highest similarity with the NP
gene from avian influenza A (H5N1) virus (A/ goose/Shantou/753/2002).
Peripheral blood samples were then collected from four patients for
quantitative analysis of GLE1
and LCP2 gene expression.
Our results demonstrated that both GLE1
and LCP2 genes were
upregulated in H5N1 influenza A virus infected patients, suggesting that
upregulation of GLE1 and LCP2 genes may be important for
the host defense against influenza A viruses.