TITLE:
Chemical Characterization and in Vitro Antitumor Activity of the Essential Oils from the Leaves and Flowers of Callistemon viminalis
AUTHORS:
Christiane Maria de Oliveira, Maria das Graças Cardoso, Marisa Ionta, Marisi Gomes Soares, Juliana de Andrade Santiago, Guilherme Álvaro Ferreira da Silva, Maria Luisa Teixeira, Danúbia Aparecida de Carvalho Selvati Rezende, Rafaela Vieira de Souza, Luana Isac Soares, David Lee Nelson, Marcos Schleiden Sousa Carvalho
KEYWORDS:
Cancer, Volatile Oil, Escova-de-Garrafa (Bottle Brush)
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.6 No.16,
October
29,
2015
ABSTRACT: The objective of this study was to characterize and verify the in vitro antitumor activity of essential
oils (EOs) extracted from the leaves and flowers of Callistemon viminalis. The EOs were extracted
by hydrodistillation using a modified Clevenger apparatus. The identification and quantification
of constituents were performed on a gas chromatograph coupled to a mass spectrometer
and a gas chromatograph with a flame ionization detector. The antitumoral activity was evaluated
by a colorimetric assay (MTS) using different cell lines derived from human tumors (breast, lung,
glioblastoma, and melanoma). The major constituents of the EOs of leaves and flowers were similar,
only quantitative differences being observed. The compounds 1,8-cineole, α-pinene and α-terpineol
were found in concentrations of 50.4%, 25.8% and 8.7% in the EOs obtained from the
leaves and 48.8%, 24.5% and 3.9% in the EOs obtained from the flowers, respectively. The cytotoxic
activity of the EOs was observed only in melanoma cultures (HT144). Cultures treated for 48
h with EOs from leaves and flowers (200 μg·mL-1) reduced the viability by 40% and 25%, respectively.
Thus, the antiproliferative activity of the EO from leaves was more pronounced than the EO
from flowers in cells derived from melanoma.