Molecular Characterization of Listeria monocytogenes Based on the PFGE and RAPD in Korea

Sanghun Park, Jihun Jung, Sungsun Choi, Younghee Oh, Jibho Lee, Heesun Chae, Seunghee Ryu, Hyowon Jung, Gunyong Park, Sungmin Choi, Bogsoon Kim, Junghun Kim, Young Zoo Chae, Byungyeol Jung, Myunghun Lee, Hyunsoo Kim

Animal Disease Diagonsis Division, National Veterinary Research and Quarantine Service, Anyang City, Korea.
College of Veterinary Medcine, Chungnam National University, Daejeon, Korea.
Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Korea.
Veterinary Pharmacology Division, National Veterinary Research and Quarantine Service, Anyang City, Korea.

DOI: 10.4236/aim.2012.24079                

Abstract

This study was performed to characterize 35 L. monocytogenes isolates from animals, foods, environmental samples collected between 1997 and 2007 with no apparent epidemiological relations, and five reference isolates using serotypic, genotypic and molecular typing methods to understand the pattern of strain distribution in Korea. For this study, we used serotyping and detected 6 different virulence-associated genes (inlA, inlB, plcA, plcB, hlyA, and actA) and 16s rRNA using multiplex-PCR. We also compared RAPD and PFGE to determine genetic characterization of L. monocytogenes strains to define the genetic diversity. Serotype patterns of the 30 L. monocytogenes strains was as follows; 9 isolates (30.0%) belonged to serotype, 7 isolates (23.3%) belonged to serotype 4b, 4 isolates (13.3%) belonged to serotype 1/2b, 3 isolates (10.0%) belonged to serotype 1/2c, 2 (6.7%) isolates belonged to4c, 2 (6.7%) isolates belonged to NT (Non Type), one isolate (3.2%) belonged to3aand 3b, and4a, respectively. Although, a limited number of isolates were analyzed in this study, molecular typing with RAPD and PFGE indicated that PFGE is more discriminatory for the subtyping L. monocytogenes than RAPD. Some L. monocytogenes isolates by RAPD and PFGE types are associated with specific sources. And, combining data obtained by these methods will increases the likelihood of strain discrimination.

Keywords

Listeria monocytogenes; PFGE; RAPD

Conflicts of Interest

The authors declare no conflicts of interest.

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