Author(s)

Mariia Boretska^1*, Iaryna Datsenko¹, Olga Suslova¹, Olena Pareniuk², Olena Moshynets³

¹General and Soil Microbiology Department, Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, Ukraine.
²Ukrainian Institute of Agricultural Radiology, National University of Life and Environmental Sciences of Ukraine, Kiev, Ukraine.
³Cell Regulatory Mechanisms Department, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev, Ukraine.

The composition of tightly bound exopolymeric substances (EPS) obtained from biofilm and plan- ktonic cell subpopulations of Stenotrophomonas maltophilia 5426 UKM was analyzed to study an impact of mild steel presence in the cultivation medium. The overall protein production was found to increase in the presence of a steel coupon. Some amino acids such as lysine, valine, iso-leucine, tyrosine and phenylalanine were found to be secreted in higher amounts in the presence of mild steel, while its absence leads to an increase of arginine, asparagine, serine, glutamine, proline, glycine, cysteine, leucine, methionine production levels. Biofilm cells EPS contained more arginine, glycine, cysteine, valine, methionine and leucine, comparing to EPS of planktonic cells. The chang- es of tightly bound EPS aggregation around the cells induced in the presence of steel coupons were revealed by transmission electron microscopy. The results suggested the transition of S. maltophilia 5426 UKM cells from planktonic to biofilm lifestyle to be a complex process involving more than a single step.

EPS, Stenotrophomonas maltophilia, Biofilm, Amino Acid Composition, Ion Exchange Chromatography, Mild Steel, Biocorrosion

Boretska, M. , Datsenko, I. , Suslova, O. , Pareniuk, O. and Moshynets, O. (2014) Amino Acid Composition of Tightly Bound Exopolymeric Substances Produced by Corrosion-Related Bacteria in Presence of Mild Steel. Advances in Microbiology, 4, 808-815. doi: 10.4236/aim.2014.412089.