TITLE:
Urinary Oxalate Excretion Decreased in Androgen Receptor-Knockout Mice by Suppressing Oxalate Synthesis in the Liver
AUTHORS:
Sayuri Takahashi, Seiji Aruga, Yoko Yamamoto, Takahiro Matsumoto
KEYWORDS:
Androgen Receptor, Urinary Stone, Oxalate
JOURNAL NAME:
Open Journal of Urology,
Vol.5 No.8,
August
19,
2015
ABSTRACT: The incidence of calcium oxalate stone in
men is higher than that in women. We evaluated the association between the
androgen receptor (AR) and urinary oxalate excretion using Crefloxed male
androgen receptor-knockout (ARKO) mice (ARL-/Y) and floxed mice (ARL+/Y) as
control. Four-teen-week-old ARL-/Y and ARL+/Y mice were fed distilled water.
Drinking water was then swapped for 0.5% ethylene glycol (EG). Urinary oxalate
was measured on days 0, 14, and 28. Urinary calcium, inorganic phosphorus,
citrate, uric acid, and ion-actibity products of calcium oxalate (APCaOx) in
mouse, AP (CaOx)-indexMOUSE, were evaluated on days 0 and 15. On day 28, livers
were harvested to measure mRNA expression of enzymes. Urinary oxalate excretion
was significantly higher in ARL+/Y than in ARL-/Y mice 14 and 28 days after EG
treatment (p L-/Y mice exhibited atrophic testes and low
serum testosterone, both ARKO and control mice were orchiectomized and
implanted DHT pellets (ARL-/Y-ORX-DHT, and ARL+/Y-ORX-DHT), and the same
experiments as above were performed. EG loading for 14 and 28 days resulted in
significantly higher excretion in ARL+/Y-ORX-DHT mice than ARL-/Y-ORX-DHT mice
(p L-1/Y-ORX-DHT mice. mRNA expression levels of glycolate oxidase
(GO) in liver were lower in ARKO mice than in control ones. AR modulates the
excretion of oxalate in urine after EG treatment, which may be associated with
increased oxalate synthesis by activated GO in the liver via the AR pathway.