Bioengineering Functional Copolymers. XVII. Interaction of Organoboron Amide-Ester Branched Derivatives of Poly(Acrylic Acid) with Cancer Cells ()
1. Introduction
Many natural polymers such as polylysine, polyarginine, dextran derivatives, heparin and chitosan, and synthetic bioengineering polymers such as poly(acrylic acid) (PAA), copolymers of maleic anhydride, have now been reported to have direct or indirect antitumor activity via stimulation of the immune system [1-3]. In recent years, the PAA and its copolymers have been often used as carriers in drug release systems, because of their multifunctional nature, unique properties and good biocompatibility [4,5]. Dimitrov et al. [5] studied the biopharmaceutical characterization of hydrogels based on crosslinked PAA and showed that this studied systems provide retarded drug release and appear to be potential candidates for use in the pharmaceutical practice. PAA is grafted to the poly(ethylene glycol) hydro-gel by photo-induced graft polymerization. Due to carboxyl functionality of PAA, collagen and cell adhesion protein, they could be covalently immobilized on to the poly(ethylene glycol) hydrogel [6]. Most of the hydrogels utilized as adhesives for dermatological patches were composed using PAA and its salts as matrix polymers. The ionic interactions between the carboxyl groups of the polymer and polyvalent cations such as calcium, copper, and aluminum cause the formation of the chemical crosslinking used to increase their mechanical strengths [7].
Acrylate-based polymers, containing carboxylic groups, exhibit a swelling behaviour depending on pH and ionic strength of solution [8,9]. Argentiere et al. [10] investigated PAA nanogels as pH-sensitive carriers for biomedical applications. They prepared PAA–biopolymer nanogels by loading and release of an oligothiophene fluorophore and its albumin conjugate onto the PAA macromolecules.
On the other hand, several synthetic boron-containing compounds exhibiting important biological properties were investigated as potential therapeutics [11,12]. The mild electrophilic nature of the boronic acid moiety has led to its use at the ‘warhead’ site of enzyme inhibitors, particularly for inhibiting proteases. For this purposes, several researchers developed some α-aminoboronic acid derivatives [11,13]. One such compound, the novel proteasome inhibitor bortezomib (Velcade) has been recently approved for clinical use as an anticancer agent for the treatment of myeloma [14].
Ban et al. [15] synthesized a series of o-carboranyl phenoxy derivatives as potent inducers for the activation of the 20S proteasome and as chemical probes for the investigation of proteasome-dependent degradation pathways. Other types of bioactive boron-containing compounds have been investigation as therapeutic agents. These include certain boron analogues of biomolecules [16], diazaborine as an antibacterial and antimalarial agent [17], various antibacterial oxazaborolidines [18], the antibacterial diphenyl borinic esters to inhibit bacterial cell wall growth [19], the antifungal agent benzoxaborole (AN2690) [20], and an oestrogen receptor modulator containing a B–N bond [21].
Some organoboron compounds, including boronic acids and its functional derivatives, and carboranes, were also investigated as agents for boron-neutron capture therapy (BCNT) for the treatment of brain tumors [12,22, 23].
The goal of this work is synthesis and characterization of novel organoboron amide-ester derivatives by amidolysis of PAA with 2-aminoethyldiphenyl borinate (2-AEPB) and their a,ω-hydroxy-methoxypoly(ethylene oxide) (PEO) macrobranched derivative by grafting of synthesized organoboron polymer with PEO to improve the biocompatibility and degree of conjugation with cancer biomacromolecules. An important aspect of this work is comparative investigations of the interactions of these novel functionalized organoboron polymers with HeLa (human cervix carcinoma cell) cancer cells and L929 Fibroblast normal cells, and evaluation of their antitumor activity (cytotoxicity, apoptotic and necrotic effects) using various biochemical methods such as hematoxylen/eosin and immune cytochemical staining, light and fluorescence inverted microscopy analyses.
Synthetic partway of the side-chain amide-, esterand carboxyl-functionalized organoboron polymers can be represented as follows (Scheme 1).
Scheme 1. Synthetic partway of the organoboron amideester-carboxyl functionalized polymers via amidolysis and esterıfication/grafting reactions.
2. Experimental
2.1. Materials
PAA (BDH) was used as 25% aqueous solution with Mw 230.000 g/mol and density 1.09 g/ml. 2-Aminoethyl diphenylborinate (2-AEPB) (Sigma-Aldrich, Germany) was purified by recrystallization from anhydrous ethanol: m.p. 193.5˚C (by DSC); FTIR-ATR spectra of 2-AEPB, cm–1: 3284 (vs) and 3220 (s) N-H stretching in NH2, 3066 (vs)-2870(s) C-H stretching, 1611(vs) NH2 bending and C=C stretching in phenyl groups, 1491(m) and 1334 (m) B-O band, 1432 (vs) fairly strong, sharp band due to benzene ring vibration in phenyl-boronic acid linkage, 1263-1154 (s) fairly strong, sharp bands due to C-N stretching in C-NH2, 1061(vs) N-H bending in NH2 and 750-710(s) sharp bands due to boron-phenyl linkage; 1H NMR spectra (δ, ppm) in CHCl3-d1: CH2-O 1.49, CH2-NH2 2.96, and 7.38-7.40 (1H), 7.19-7.24 (2H) and 7.13-7.16 (2H) for protons of p-, oand m-positions in benzene ring, respectively.
a-Hydroxy-ω-methoxy-PEO (Mn 2000 g
mol–1) (Fluka). 1H NMR spectra (δ, ppm) in CHCl3-d1: CH2-O3.75-3.45, OH end group 2.61 and O-CH3 end group 2.16.
N-Ethyl-N-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) as a catalyst and folic acid (FA) as a targeting agent were supported from Aldrich-Sigma (Germany). All solvents and reagents were of analytical grade and used without purification HeLa (human cervix carcinoma cell) cancer cells and L929 Fibroblast cells were obtained from the tissue culture collection of the SAP Institute (Ankara, Turkey). Cell culture flasks and other plastic material were purchased from Corning (NY, USA). The growth medium, which is Dulbecco Modified Medium (DMEM) without L-glutamine supplemented fetal calf serum (FCS), and Trypsin-EDTA were purchased from Biological Industries (Kibbutz Beit Haemek, Israel). The primary antibody, caspase-3 was purchased from Lab Vision (Germany).
2.2. Synthesis of 2-Amidoethyldiphenylborinate-poly(Acrylic Acid)
Amidolysis of PAA with 2-AEPB using various [PAA]/[2-AEPB] mole ratios was carried out in N,N’-dimethylformamide (DMF) at 60˚C with EDAC catalyst under the nitrogen atmosphere using a standard Pyrex-glass reactor supplied by a mixer, temperature control unit and condenser. Reaction conditions: [2-AEPB] = 0.066 mol
L–1, mole ratios of [PAA]/ [2-AEPB] = 1: 1, 3: 1, 5: 1 and EDAC = 1.0 wt %. Appropriate quantities of PAA, 2-AEPB, DMF and EDAC were placed in a reactor and the reaction mixture was flushed with dried nitrogen gas for at least 2 min, then sealed and placed in a thermo stated silicon oil bath at 60˚C to intensive mixing for 5 h. The organoboron amide polymer was isolated from reaction mixture by precipitation with diethyl ether and dried under vacuum. Synthesized organoboron polymer has the following average parameters: Tg (by DSC) 190.6˚C, [h]in in deionized water at 25˚C 2.72 dL
g–1; FTIR-ATR spectra of PAA-g-2-AEPB (KBr pellet), cm–1: 1702 (m) C=O stretching (amide I band), 1642 (w) and 1542 (m) N-H deformation (amide II band), 1446(m) and 1407(w) C-N stretching (amide III band); 1H NMR spectra (in DMSO-d6 at 25˚C) δ ppm: protons of phenyl groups 7.9, 2H from CH2 in -CH2-CO-NHfragment 7.3, 2H from B-O-CH2 group 3.6, 2H from NH-CH2 group 3.0 and 2H from backbone -CH-CH- 3.3, 2.2 and 1.2-1.7; 13C NMR spectra (δ ppm): C=O of PAA unit/amide linkage 177, CH= in phenyl groups 162-158, backbone CH2 and CH128-127 and 57, NH-CH2 41-42, CH2-O 31-36.
2.3. Synthesis of PEO Branched Organboron Copolymer
The esterification (grafting) of organoboron amide of PAA, containing 19.24 mol % of organoboron groups, with PEO (Mn 2000 g
mol–1) at organoboron polymer/PEO feed molar ratio 1: 0.01 was carried out in DMF at 60˚C for 1 h. PEO branched polymer was isolated from reaction mixture by precipitation with diethyl ether and dried 40˚C under vacuum. Prepared PEO ester of organoboron polymer has the following average characteristics: Tg 175.8˚C (by DSC); [h]in in deionized water at 25˚C 0.9 dL
g–1; 1H NMR spectra (in DMSO-d6 at 25˚C) δ ppm: protons of phenyl groups 7.8, CH2 in CH2-CO-NHamide group 7.2, 2H in B-O-CH2 3.5, CH2CH2 in PEO branch 3.35, 3H in OCH3 end group 3.1 and 2H in NH-CH2 2.9; 13C NMR spectra (δ ppm): C=O in –COOH 176, CH= in phenyl groups 162, backbone CH2 and CH 128 and 127, respectively, O-CH2 in PEO 69, end OCH3 group of PEO 57, NH-CH2 41-42, and CH2-O-B 31-36.
2.4. Characterization
Fourier transform infrared (FTIR-ATR) spectra were recorded with FTIR Nicolet 8700 spectrometer in the 3700 - 600 cm–1 range. 1H and 13C NMR spectra were performed on a Bruker Avance (300 MHz) spectrometer with DMSO-d6 as a solvent at 25˚C. Thermo gravimetric (TGA) and differential scanning calorimetric (DSC) analyses were performed in a TGA-DTA (Perkin Elmer TG/DTA6300) and a DSC2010 Thermal Analyzers, respectively, under nitrogen atmosphere at a heating rate of 10˚C/min. The intrinsic viscosity [h]in values of the organoboron polymers were determined in deionized water at 25˚C ± 0.1˚C in the concentration range 0.003 - 0.06 g
dL–1 using an Ubbelohde viscometer.
Analyses of cytotoxicity, apoptotic and necrotic cells with double staining and immune cytochemical stains of the synthesized novel organoboron functional polymers were performed according to the modified methods using in our recent published work [25,26]. For cytotoxicity experiments, HeLa and L 929 Fibroblast cells (50 × 103 cells per well) were placed in DMEM by using 24-well plates. Different amounts of pristine PPA and organoboron polymers (PAA-B, PPA-PEO-B and PAA-PEO-B-F) (about 0-650 mg
mL–1 in aqueous solutions) were put into wells containing cells, respectively. The plates were kept in the CO2 incubator (37˚C in 5% CO2) for 24 h; the medium was replaced with fresh medium, and incubated at the same conditions for 24 h. Following of this incubation, HeLa cells were harvested with trypsin–EDTA, and then were dyed with trypan blue. The number of living and dead cells were counted with a haemacytometer (C.A. Hausse & Son Phluila, USA), using light microscope at ×200 magnification. Analysis of apoptotic and necrotic cells with double staining were performed to quantify the number of apoptotic cells in culture on basis of scoring of apoptotic cell nuclei. HeLa and L929 fibroblast cells (20 × 103 cells per well) were placed in DMEM by using 24-well plates. After treating with different amount functional oligomers (about 0 - 650 mg
mL–1 in aqueous solutions) for 24 hours period, both attached and detached cells were collected, then washed with PBS and stained with Hoechst dye 33342 (2 mg
mL–1), propodium iodide (PI) (1.0 mg
mL–1) and DNAse free-RNAse (100 mg
mL–1) for 15 min at room temperature. After that 10 - 50 mL of cell suspension was smeared on slide and cover slip for examination by fluorescence microscopy. The nuclei of normal cells were stained light blue but apoptotic cells were stained dark blue by the Hoechst dye.
The apoptotic cells were identified by their nuclear morphology as a nuclear fragmentation or chromatin condensation. Necrotic cells were staining red by PI. Necrotic cells lacking plasma membrane integrity and PI dye cross cell membrane, but PI dye don’t cross non necrotic cell membrane. The number of apoptotic and necrotic cells in 10 randomly chosen microscopic fields were counted and the result expressed as a ratio of apoptotic and necrotic to normal cells. The number of apoptotic and necrotic cells were determined by light and fluorescence inverted microscope (Leica, Germany). The cell images were also recorded using the both above mentions microscopes with DAPI and FITC filters, respectively.
For immunocytochemical stains, about 2 ml HeLa (20 × 103 cells per well), treated with functional oligomers (about 0 - 650 mg
mL–1 in aqueous solutions) suspension was centrifuged for 5 min in a Hettich centrifuge. Cytospin preparations were fixed in 70 % ethanol for immunocytochemistry.
For an indirect immunocytochemical procedure, cytology specimens were treated with 3% H2O2 for 10 min, taken to water, and then rinsed in PBS (pH 7.4) for 5 min. Nonspecific protein binding was blocked on specimen by incubating with blocking solution for 10 min. The primary antibody, caspase-3 (Lab Vision) used at 1:300 dilution, incubated for 1 h at room temperature. Specimens were washed with PBS buffer (pH 7.4) and incubated in biotinylated secondary antibody solution for 10 min. Diaminobenzidine (Dako) served as the chromagen and Mayer’s hematoxylin as the counter stain. For the negative control the primary antibody was omitted in one of the slides.
The immunocytochemical staining results were controlled independent and blindly by observers without the knowledge of treatment. The immunoreactivity of the caspase-3 antibody is confined to the cytoplasm of apoptotic cells. We counted the number of caspase-3-positive cytoplasmic staining cells in all fields found at ×400 final magnification. For each image, three randomly selected microscopic fields were observed, and at least of 100 cells/field were evaluated.
3. Results and Discussion
3.1. Structure of Organoboron Polymers
The structures of synthesized organoboron polymers and their PEO branches were confirmed by FTIR-ATR and 1H (13C) NMR analysis. Comparative analysis of FTIR-ATR spectra (Figure 1) of 2-AEPB, PAA and its organoboron derivative indicates that the characteristic bands of acid C=O groups disappearance in the spectra of PAA-B-1 polymer prepared from 1:1 molar feed ratio of PAA: 2-AEPB.
The formation of amide bound in this organoboron polymer is confirmed by the appearance of new bands such as 1702 (amide-I band), 1642 and 1542 (amide-II band), 1446 cm–1 and 1407 (amide-III band). Simultaneously a very broad band between 3500 and 2500 cm–1 appearances in spectra due to increase hydrogen bonded fragments in organoboron polymer (PAA-B-1). As the intensities of amide bands significantly decrease in spectra of PAA-B-2 and PAA-B-3 polymers prepared from
Figure 1. FTIR spectra: (1) 2-AEPB, (2) PAA and (3) PAAg-2-AEPB-1 organoboron amide polymer.
PAA/ 2-AEPB mixtures enchasing with PAA (PAA>> 2-AEPB), the intensities of free acid group bands increase. Similar effect has been observed from comparative analysis of the 1H and 13C NMR spectra of A-B-2and its PEO branch (PAA-B-PEO). The results of this analysis are illustrated in Figures 2 and 3. The formation of H-bonded amide linkages is confirmed by a presence of characteristic broad peaks at 7.3 and 177 ppm in the 1H and 13C NMR spectra of PAA-B-2, respectively (Figure 2). In addition, the presence of characteristic proton peaks of organoboron linkages such as quarter phenyl peak at 7.9 ppm, triplet B-O-CH2 peak at 3.6 ppm andquarter NH-CH2 peak at 3.0 ppm (Figure 2(a)) also confirmed that 2-AEPB is covalently bound to anhydride units. In the 13C NMR spectra of PAA-B-2 polymer (Figure 2(b)), the characteristic carbon resonances (162, 158, 41, 42, 31 and 36 ppm) from organoboron fragment are also observed.
(a)
(b)
Figure 2. (a) 1H NMR and (b)13C NMR spectra of organoboron polymer (PAA-B) in DMSO-d6.
(a)
(b)
Figure 3. (a) 1H NMR and (b) 13C NMR spectra of PAA-gAEPB-2-g-PEO in DMSO-d6.
1H (13C) NMR spectra of PEO grafted organoboron PAA polymer (PAA-B-PEO) were illustrated in Figure 3. The observed proton signals from of side-chain PEO branches at 3.4 and 3.1 ppm for (CH2-CH2-O)n units (Figure 3(a)) and carbon atom resonances (69 ppm for O-CH2 and 57 ppm for OCH3 end group) (Figure 3(b)) may be served an additional fact to confirm the formation of side-chain macrobranched PEO linkages.
3.2. Functional Polymer Composition–Property Relationship
The results of intrinsic viscosity measurements from the plots of hsp/c (specific viscosity) vs. c (polymer concentration in deionized water) for the organoboron PAA and PEO derivatives having different compositions are illustrated in Figure 4. A visible decrease of hin value with
Figure 4. The plots of ηsp/c (specific viscoity) versus c (polymer concentrations in deionized water) for the determination of intrinsic viscosity and evaluation of polymer composition-viscosity relationships (dilution effect and polyelectrolyte behaviour): –□– PPA-B-1, –·– PPA-B-2, –o– PAA-B-3 and –■– PAA-B-PEO.
Table 1. Some characteristic parameters of organoboron amide (PAA-B) and PEO branched (PAA-B-PEO) derivatives of PAA.
increasing the organoboron fragment in PAA polymer was observed (Table 1). Unlike the PEO branched derivative the organoboron polymers exhibit typical polyelectrolyte behavior, i.e., increase in viscosity with a dilution of polymer water solution, which can be explained by specific behavior of complexed macromolecules and their conformational changes resulting in the expansion of polymer coil in the dilution solution. Similar effect was observed for the other carboxyl-containing polymers [27,28].
Thermal behavior and phase transitons of synthesized organoboron polymers were investigated by differential scanning calorimetric (DSC) and thermal gravimetrical analysis (TGA) methods. The obtained results were summarized in Figure 5. It was found that PAA and its organoboron and PEO branched derivatives exhibit amorphous structure with characteristically broad endo-peaks, which are associated with the glass-transition temperatures (Tg), significantly depend on the composition and content of organoboron linkages in the functional polymers. The higher values of Tg are observed for the polymers containing relatively high organoboron linkages.
Figure 5. DSC and TGA curves of functional organoboron polymers: (1) PAA-B-1, (2) PAA-B-2, (3) PAA-B-3, (4) PAA-B-PEO and (5) pristine PAA. Heating rate 10˚C /min under a nitrogen atmosphere.
Therefore, rigid H-bonded structure provides high Tg in the organoboron polymers (curves 1-3). The results of TGA analyses (Figure 5) indicate that the organoboron polymer and PEO branched derivative of PAA show higher thermal stability which increases with increasing degree of grafted organoboron linkage in the polymer. The observed two step degradation of the PAA and its functionalized derivatives indicates occurrence of some macromolecular reactions. TGA analyses also allow us to determine the content of boron in studied functionalized polymers, results of which are summarized in Table 1.
3.3. Cytotoxicity
The obtained cytotoxicity results of the pristine PAA and its organoboron amide (PAA-B) and organoboron amide-ester (PAA-B-PEO) branches, and PAA-BPEO/ folic acid complex (PAA-B-PEO-F) on cancer cells using a trypan blue staining were illustrated in Figure 6. As seen from plots of concentration of polymers versus percent of cell viability, the toxicity of pristine PAA against cancer and normal cells decreased with an increasing in polymer concentration from 100 to 200mg
mL–1 for 24 h incubation at 37˚C. If polymer concentration was higher than 20 mg
mL–1, its toxicity increased, especially higher toxicity exhibits for 24 h incubation. The toxicity of PAA-B (organoboron amide polymer) was more significant than other polymer systems.
Figure 6 shows that the number of viable cells is above 80% for normal and cancer cells after incubation