TITLE:
Molecular Characterization, 3D Modeling of Grass Carp Interleukin-10 Receptor 1 (IL10R1)
AUTHORS:
He Wei, Shangnian Wang, Lei Qin, Xinyan Wang, Hong Zhou
KEYWORDS:
Grass Carp; Interleukin-10 Receptor 1; Structure Characterization; 3D Modeling
JOURNAL NAME:
Engineering,
Vol.5 No.10B,
October
31,
2013
ABSTRACT:
Interleukin-10 (IL-10) is an important cytokine that
plays a pivotal role in natural and adaptive immune systems. However, in lower
vertebrates, especially in teleost the receptor of this cytokine is still
largely unknown. This paper described the cloning and characterization of grass
carp interleukin-10 receptor 1 (gcIL10R1) and the 3D structure of its
extracellular domain was predicted. The gcIL10R1 cDNA included 180 bp5’ untranslated region (UTR), 870 bp3’ UTR and an open reading frame (ORF) of
1632 bp. The ORF was found to encode a 543 amino acid protein with a putative
JAK1 binding site, one STAT3 binding site. The phylogenetic analysis clusters
gcIL10R1 with other teleost IL10R1s but independently of the amphibian, avian
and mammalian IL10R1s. The 3D structure of its extracellular domain was the
first homology model of a fish IL10R1 that revealed a high similarity with its
mammalian and avian counterparts.