TITLE:
Fungitoxicity of Trichoderma longibrachiatum (Rifai) Metabolites against Fusarium oxysporum, Aspergillus niger and Aspergillus tamarii
AUTHORS:
Ayodele Adegboyega Sobowale, Linda Chidinma Uzoma, Adefoyeke Olufunmilayo Aduramigba-Modupe, Bukola Ayodeji Bamkefa
KEYWORDS:
Antifungal Metabolites, Trichoderma longibrachiatum, Fusarium oxysporum, Aspergillus niger, Aspergillus tamari
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.13 No.7,
July
22,
2022
ABSTRACT: An experiment was carried out to evaluate the
fungitoxic effect of Trichoderma
longibrachiatum (Rifai) metabolite on F. oxysporum, A. niger and A. tamarii.
The fungi were collected from the International Institute of Tropical Agriculture (IITA) and Nigerian Institute of Science
Laboratory Technology (NISLT). T. longibrachiatum was cultured on 1/4 strength Potato Dextrose Broth (PDB) following
standard procedures. Its metabolite was extracted using 50 ml n-hexane with 50 ml
Potato Dextrose Broth (PDB). The metabolite was purified by
filter sterilization using a sterile 0.22 millipore
filter disc after centrifuging at 900 rpm for 20 minutes. Petri plates of each fungus were later impregnated in triplicates
with the T. longibrachiatum metabolites using four concentrations (10%, 25%, 50% and 100%), and three
volumes (1 ml, 2 ml, and 3 ml). Petri plates of fungi without the metabolite and Petri
Plates of fungi with n-hexane and PDB served as control. All Petri
Plates were incubated at 28°C for 7 days. Radial and diametric growth of
each fungus on all Petri Plates were taken daily at 24 hours intervals. Data obtained were
analysed using SAS (version 9.3). Growth inhibition of F. oxysporum, A. niger and A. tamarii was significantly higher than in control in that order (P
≤ 0.05). Inhibition of the fungi by metabolites extracted with both PDB and
n-hexane was significantly better than in control. Generally, inhibition by
metabolite extracted with PDB was better than that extracted with n-hexane.
Growth inhibition at all the concentrations of the metabolite was significantly
better than in the control (P ≤ 0.05). Generally, inhibition at 100%, 50% and 25%
concentrations was better than that at 10% concentration of the metabolite. Trichoderma longibrachiatum metabolite
can thus be said to possess promising fungitoxic potential even at concentrations as low as 25%. Both PDB and
n-hexane are good extraction media for the metabolite.