TITLE:
Investigation on Viral Pathogens in Vitis vinifera from Four Production Bases in Hangzhou Vicinity of China by sRNAseq and Molecular Validation
AUTHORS:
Hongmei Li, Lingzhu Wei, Jiang Wu, Nongnong Shi
KEYWORDS:
Vitis vinifera, Viral Disease, Small RNA Deep Sequencing, RT-PCR
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.12 No.12,
December
22,
2021
ABSTRACT: This is the first systematic investigation of viral
pathogens in Vitis vinifera from Hangzhou vicinity of China. About 7 viruses and 5 viroids were annotated
from four production bases “Dushicun”, “Wangjiayuan”, “Xiajiangcun”, and
“Yangducun” covering 15 cultivars through sRNAseq technique. At least 3 viruses—grapevine leaf roll-associated virus 3 (GLRaV-3),
grapevine fleck virus (GFkV) and grapevine
geminivirus A (GGVA), and 4 viroids—hop stunt viroid (HSVd), citrus
viroid II (CVd-II), grapevine yellow speckle viroid 1 (GYSVd-1) and grapevine
yellow speckle viroid 2 (GYSVd-2) infected all four bases. “Yangducun” base
showed 11, the most infected pathogens. GYSVd-1 showed the highest accumulation
in host of Wangjiayuan base. The main infected
pathogens were verified by reverse-transcription polymerase chain reaction (RT-PCR) technique, the detected rate reached to 85% - 100%. The results
provide an important basis for effective and precise detection of viral
diseases in the area and for the virus-free cultivation in future.