Li, H.M., Wei, L.Z., Qin, C., Cheng, J.H., Zhang, X., Chen, W.W., Lai, T.F., Yu, Z.M., Zhang, P.C., Wu, J., Hong, Y.G. and Shi, N.N. (2021) Characterization of Viruses and Viroids in Vitis vinifera “Kyoho” in Hangzhou China by Small RNA Deep Sequencing and Molecular Detection. Journal of Horticultural Science & Biotechnology, 96, 400-406.
https://doi.org/10.1080/14620316.2020.1845985

has been cited by the following article:

• TITLE: Investigation on Viral Pathogens in Vitis vinifera from Four Production Bases in Hangzhou Vicinity of China by sRNAseq and Molecular Validation

  AUTHORS: Hongmei Li, Lingzhu Wei, Jiang Wu, Nongnong Shi

  KEYWORDS: Vitis vinifera, Viral Disease, Small RNA Deep Sequencing, RT-PCR

  JOURNAL NAME: American Journal of Plant Sciences, Vol.12 No.12, December 22, 2021

  ABSTRACT: This is the first systematic investigation of viral pathogens in Vitis vinifera from Hangzhou vicinity of China. About 7 viruses and 5 viroids were annotated from four production bases “Dushicun”, “Wangjiayuan”, “Xiajiangcun”, and “Yangducun” covering 15 cultivars through sRNAseq technique. At least 3 viruses—grapevine leaf roll-associated virus 3 (GLRaV-3), grapevine fleck virus (GFkV) and grapevine geminivirus A (GGVA), and 4 viroids—hop stunt viroid (HSVd), citrus viroid II (CVd-II), grapevine yellow speckle viroid 1 (GYSVd-1) and grapevine yellow speckle viroid 2 (GYSVd-2) infected all four bases. “Yangducun” base showed 11, the most infected pathogens. GYSVd-1 showed the highest accumulation in host of Wangjiayuan base. The main infected pathogens were verified by reverse-transcription polymerase chain reaction (RT-PCR) technique, the detected rate reached to 85% - 100%. The results provide an important basis for effective and precise detection of viral diseases in the area and for the virus-free cultivation in future.