TITLE:
Efficacy of a Biorevitalizing-Filler Solution on All Skin Aspects: 10 Years Approach through in Vitro Studies and Clinical Trials
AUTHORS:
Sophie Robin, Férial Fanian, Carol Courderot-Masuyer, Michel Tordjman, Frederic Braccini, Sylvie Boisnic, Valérie Philippon, Anne Grand Vincent, Catherine Salomon, Michel Manfait, Philippe Humbert, Philippe Piccerelle
KEYWORDS:
Mesotherapy, Skin Rejuvenation, NCTF 135HA®, Hyaluronic Acid, Skin Bio-revitalization, Skin Booster
JOURNAL NAME:
Journal of Cosmetics, Dermatological Sciences and Applications,
Vol.11 No.1,
March
8,
2021
ABSTRACT: Introduction: Skin aging is the result of many cellular dysfunctions over time
particularly the fibroblasts and the keratinocytes. These dysfunctions could be
decelerated by the preventive effects of some skin treatments such as Intradermal
injections. NCTF 135HA has a polycomponent formulation designed to improve the efficacy of
non-cross linked hyaluronic acid (as a micro-filler) on fibroblasts function.
Although NCTF 135HA has been used by aesthetic practitioners since 20 years, we
have analyzed all in vitro, ex vivo and in vivo studies during past 10 years in order to summarized its anti-aging effect. Methods: In these series of studies, the known effects of HA have been evaluated with this
boosted formula. Collagen I synthesis, antiglycation effect and contractile
forces developed by fibroblasts were studied. Fibroblasts and keratinocytes
proliferations were evaluated in monolayer cells culture. The filling effect
thanks to NCTF 135HA injection was ex
vivo performed by fringe projection and the protective effect of NCTF 135HA
against solar irradiation was performed ex
vivo to study proliferation rate, elastin and collagen expression. Two
clinical trials were performed on women to evaluate the anti-aging effect of
NCTF 135HA injection. Hydration, firmness, radiance, wrinkles size, pore size,
thickness and density of the dermis were analyzed. Results: Collagen I, anti-AGE products, cell proliferation and contractile forces
were significantly increased with NCTF 135HA in culture medium. Ex vivo studies showed that NCTF 135HA
increased, cells proliferation rate, elastin and collagen production. In
addition, the filling effect of the NCTF 135HA is significantly superior to
placebo. The first clinical trial shows that NCTF 135HA injection significantly
increased tonicity, hydration and radiance of the skin and significantly
decreased wrinkle score. The second clinical trial demonstrated that NCTF 135HA
injection significantly increased dermis density and thickness, skin
homogeneity and skin radiance and significantly decreased pore size and wrinkle
depth and volume. Conclusion: In vitro, ex vivo and in vivo studies showed that this minimally invasive technique could be used in order to
improve the skin quality which could have a visual effect on preventing the
aging signs.