TITLE:
Continuous Fermentation of a Prodigiosin-Producing Serratia marcescens Strain Isolated from Soil
AUTHORS:
Qi Fu, Yujuan Xiao, Xuehui Duan, Huabin Huang, Zhixia Zhuang, Jinhai Shen, Yangyue Pei, Haojia Xu, Meiyu Gan
KEYWORDS:
Serratia marcescens, Prodigiosin, Foam, Continuous Fermentation, Automatic Concentration
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.10 No.4,
April
26,
2019
ABSTRACT: A red-pigmented
bacterial strain NS-17 was isolated from soil, and physiological and
biochemical characteristics and 16S rDNA analysis established this organism as
a strain of Serratia marcescens. The
red pigment isolated from cells of NS-17 was identified as prodigiosin. By
analyzing factors affecting the production of prodigiosin, modified medium and
culture conditions were set up, and a continuous fermentation method was
carried out to take advantage of the mass production of foam during
fermentation. Continuous fermentation was achieved in the following conditions:
32℃, 1:1 ventilation ratio, and medium with a nutrient concentration 5-fold
higher than that of the fermentation medium (with half the inorganic salt
concentration) supplied as a feed medium at a flow rate of 8 mL/min. For the
first time of continuous fermentation of Serratia
marcescens coupling with foam flotation, a high yield of prodigiosin was obtained.
After 56 h of culturing, the total harvest of cells was enhanced 2.33-fold
compared to that of batch fermentation, the total amount of prodigiosin was
enhanced 2.70-fold compared to that of batch fermentation, and cells and prodigiosin
were concentrated in the efflux broth automatically.