TITLE:
Utilizing Short Tandem Repeats (STRs) as a Resolving Matrix in Parental Dispute DNA Analysis
AUTHORS:
George Gborienemi Simeon, Alade Tolulope Olukemi
KEYWORDS:
Short Tandem Repeats, Matrix, Parental, DNA Analysis
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.8 No.3,
June
11,
2018
ABSTRACT: Interest in DNA analysis using short tandem repeats
(STR) as finger printing tools in forensic medicine has gained tremendous
application, as expression of these nuclear factors have enhanced forensic
examination. Here we used this Biochemical characterization after conventional
extraction process, polymerase chain reaction (PCR), gel electrophoresiss and a
sequencer to distinguish and resolve parental dispute. The differential
migration of labeled DNA fragments which attains excitation energy with a laser
elicits fluorescent light of different wavelength depending on the dye used. A
data collection software (Genemapper) collects raw data (spectrograph) and
converts it to an electropherogram that is interpreted. By comparing the DNA
profiles, inclusion and exclusion criteria were elucidated to resolve disputes.
The inherent discriminating power of STRs used in analysis enhances resolution
of cell mixtures, genetic aberration, substantiation of tissue origin and
provides genetic distinction which is a robust and reliable
approach in resolving parental disputes.