TITLE:
Analysis of Two Clonal Lines (Embryogenic and Non-Embryogenic) of Agave fourcroydes Using AFLP and MSAP
AUTHORS:
Kelly M. Monja-Mio, Adriana Quiroz-Moreno, Gastón Herrera-Herrera, Jorge Luis Montero-Muñoz, Felipe Sánchez-Teyer, Manuel L. Robert
KEYWORDS:
Agave, Clonal Line, Somatic Embryogenesis, DNA Methylation
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.9 No.4,
March
19,
2018
ABSTRACT: Somatic
embryogenesis is a very efficient way to propagate economically important
plants; however, not all genotypes within a species can be propagated using
this method, as a combined effect of both genetic and epigenetic mechanisms may
be involved in the response. The aim of the present study was to perform a
comparative analysis of the genetic differences through amplified fragment length polymorphism (AFLP)
and the epigenetic differences through methylation-sensitive amplified polymorphism (MSAP)
of two Agave fourcroydes clonal
lines, one highly embryogenic (K33) and the other non-embryogenic (K7). Genetic
and epigenetic variabilities existed within each clonal line;
however, the polymorphic profiles from the two marker systems allowed us to clearly
distinguish the two clonal lines before somatic embryogenesis induction. During
the induction, the changes detected were mainly 1) unmethylated fragments in
the initial explants that were methylated during induction (methylation events)
and 2) fragments with different methylation states in the initial explant that
were unmethylated in some stages of the process (demethylation events). K33
showed greater dynamism in relation to methylation/demethylation events, while
K7 presented the methylation events in a more constant range and at higher
levels during all process.