TITLE:
Effect of Low-Sodium, Choline-Based Semen Diluent on Viability of Bovine Spermatozoa Stored at 4°C
AUTHORS:
Pedro A. Delgado, Toby D. Lester, Rick W. Rorie
KEYWORDS:
Semen Extender, Bovine Sperm, Refrigerator Storage
JOURNAL NAME:
Advances in Reproductive Sciences,
Vol.6 No.1,
February
27,
2018
ABSTRACT: This study evaluated sperm viability over time, after dilution and refrigerator storage of fresh semen extended in either synthetic cauda epididymal plasma (CEP2), or in a low sodium medium (CJ2) supplemented with either AlbuMAX or egg yolk. Semen collected weekly for 4 weeks from 4 bulls and assigned within bulls, across treatments. After extension in either CEP2, or CJ2 containing either egg yolk or AlbuMAX, semen was cooled to 4°C, and evaluated for 7 days. A computer assisted sperm analysis (CASA) system was used for sperm evaluation. Particular emphasis was placed on sperm motility since it is the single most important sperm parameter influencing bull fertility. Total and progressive motility of sperm in CEP2 and CJ2-AlbuMAX were similar (P = 0.85 and P = 0.23, respectively), but both were lower (P