TITLE:
Applicability of Dimedone Assays for the Development of Online Aldehyde Sensor in Seawater Flooding Systems
AUTHORS:
Mohammed A. Al-Moniee, Cees Koopal, Naim Akmal, Sjaak van Veen, Xiangyang Zhu, Peter F. Sanders, Peter F. Sanders, Fahad N. Al-Abeedi, Ayman M. Amer
KEYWORDS:
DNA Staining, Automated Monitoring, Autonomous Microbe Sensor, Microbial Sensor Prototype, Injection Seawater
JOURNAL NAME:
Journal of Sensor Technology,
Vol.6 No.4,
October
24,
2016
ABSTRACT: Biocides are oilfield chemicals that are widely used to control bacterial activity
throughout the oil industry. A feasibility study has been explored to develop detection
techniques for biocide batch treatments, preferably on-line and in real-time, for
their potential use in seawater flooding system. Several methods to measure key
components of the biocide formulation were investigated and reported in previous
study [1]. The enzymatic activity of an immobilized acetylcholine esterase (AChE)
on the column material was successfully inhibited by some model compounds, but
not by the actual biocides commonly used in Saudi Aramco seawater flooding system.
In this paper, an alternative assay for biocide detection in the Saudi Aramco
seawater flooding system was investigated for its applicability for the development of
on-line biocide sensor. The assay was based on the detection of aldehyde functionality
in the biocide mixture through measurement of a fluorescent derivative formed in
the reaction of aldehyde groups and dimedone in the presence of ammonium acetate.
The reaction of aldehyde groups with dimedone was demonstrated in seawater matrix,
and the formed fluorescent product was successfully measured. The results
showed that the dimedone-based assay was very sensitive, and relatively straightforward
to perform. The ruggedness test also indicated that the assay is sensitive to minor
changes of various specific conditions of the method. It is concluded that the
dimedone assay is suitable for further development of a real-time biocide monitoring
system to detect the presence of biocide slugs in seawater flooding system. The development
of an automated on-line biocide sensor based on dimedone assay is underway.