TITLE:
Cultivable Sponge-Associated Actinobacteria from Coastal Area of Eastern Mediterranean Sea
AUTHORS:
Özlem Öner, Güner Ekiz, E. Esin Hameş, Volkan Demir, Özkan Gübe, F. Can Özkaya, M. Baki Yokeş, Ataç Uzel, Erdal Bedir
KEYWORDS:
Sponge-Associated Actinobacteria, Streptomyces, Nocardiopsis, Antimicrobial Activity
JOURNAL NAME:
Advances in Microbiology,
Vol.4 No.6,
May
13,
2014
ABSTRACT:
In this study, 16 marine sponge samples collected from Eastern
Mediterranean Sea were used for the isolation of sponge-associated Actinobacteria. Different media and
pre-treatment methods were applied for the isolation experiments. In total,
7578 actinomycetes colonies were observed on the primary isolation plates, and
20 representative isolates were selected for further studies based on their
morphologies and chemical profiles. These isolates were investigated for their
antimicrobial activities, seawater requirements and 16S rRNA gene sequences.
The phylogenetic analysis revealed that 80% of the isolated Actinobacteria was a member of the Streptomyces genus. All the isolates
showed no absolute requirement for seawater. Fifteen of the selected isolates
exhibited antimicrobial activity against methicillin resistant Staphylococcus aureus and vancomycin
resistant Enterococcus faecium,
whereas 3 isolates were active against Candida
albicans.