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has been cited by the following article:

• TITLE: Expression of Telomerase Reverse Transcriptase during the Malignant Transformation of Cadmium-Induced Cells

  AUTHORS: Min Wang, Yixiong Lei

  KEYWORDS: Cadmium Chloride; Human Bronchial Epithelial Cells; Malignant Transformation; Telomerase Reverse Transcriptase

  JOURNAL NAME: Journal of Geoscience and Environment Protection, Vol.2 No.2, April 21, 2014

  ABSTRACT: The objective of the present study was to investigate human telomerase reverse transcriptase (hTERT) mRNA and protein expressions during the cadmium chloride-induced malignant transformation of human bronchial epithelial (16HBE) cells. Fluorescence quantitative PCR (FQ-PCR) and Western blot analyses were performed to detect the hTERT mRNA and protein expressions in normal 16HBE cells, cadmium chloride-transformed 16HBE cells, and tumorigenic cells from nude mice inoculated with cadmium chloride-transformed 16HBE cells. Under the inner standard of GAPDH, the hTERT mRNA expression was significantly higher at different stages of malignant transformation (cadmium chloride-transformed 16HBE cells at passages 15 and 35 and tumorigenic cells from nude mice) than in normal 16HBE cells, and increased with the development of malignancy (P