TITLE:
Transcriptome profiling of 6-OHDA model of Parkinson’s disease
AUTHORS:
Maria I. Shadrina, Elena V. Filatova, Anelya Kh. Alieva, Alla V. Stavrovskaya, Rudolph M. Khudoerkov, Svetlana A. Limborska, Sergei N. Illarioshkin, Petr A. Slominsky
KEYWORDS:
6-OHDA; Parkinson’s Disease; Transcriptome
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.6A,
June
20,
2013
ABSTRACT: Analysis
of monogenic forms and candidate genes of Parkinson’s disease (PD) does not
allow to describe completely the contribution of genetic factors to the
etiopathogenesis of the disorder. An approach associated with an analysis of
changes in a transcriptome pattern during the development of the disease in
model objects can be used to identify new candidate genes that are involved in
the pathogenesis of PD. In this work, we performed a transcriptome analysis of
a PD model, created via stereotaxic unilateral introduction of the
6-hydroxidopamine (6-OHDA) into the substantia nigra pars compacta (SNpc) of a
rat brain, to identify new candidate genes for PD. We studied transcriptome alterations
in the substantia nigra of the rat brains 2 weeks after toxin administration,
when the rats developed the Parkinson-like phenotype, and 4 weeks after toxin
administration, when maximal changes in the behavior of animals were observed.
The transcriptome analysis of the substantia nigra of the rat brains at the
first time point (2 weeks) revealed changes in expression of genes that were
clustered with high significance (p