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has been cited by the following article:

• TITLE: DNA fingerprinting of Bacillus cereus from diverse sources by restriction fragment length polymorphism analysis

  AUTHORS: Swarnakaran Hemalatha, Narasimhan Banu

  KEYWORDS: Bacillus Cereus; Antibiotic Resistance; Heavy Metal Tolerance; RFLP; DNA Finger Printing

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.1 No.2, June 30, 2010

  ABSTRACT: Bacillus cereus is an opportunistic pathogen causing food poisoning manifested by diarrhoeal or emetic syndrome. It is closely related to animal and human pathogens Bacillus anthracis and the insect pathogen Bacillus thuringiensis. In the present study, antibiotic resistance, heavy metal tolerance & molecular typing of Bacillus cereus from diverse sources such as soil, sewage water, air, fresh water, sea water and milk were studied. Bacillus cereus resistant to Penicillin (10 units/ml) and Amoxycillin (10 µg/ml); sensitive to Cobalt and Mercury even at 1 mM concentration were determined. All the isolate had plasmid ranging in number from 3-5 and their molecular weight ranged from 3.0 kb-2.0 kb. Plasmid DNA band specific to 6,557 bp has been found in Bacillus cereus isolated from sewage water, air, fresh water, sea water and milk.