TITLE:
Comparison of the Bacterial Microbiota in a Bale of Collected Cardboard Determined by 454 Pyrosequencing and Clone Library
AUTHORS:
Valérie Lalande, Simon Barnabé, Jean-Charles Côté
KEYWORDS:
454 Pyrosequencing, Bacterial Microbiota, Cardboard, Clone Library, Paper Mill
JOURNAL NAME:
Advances in Microbiology,
Vol.4 No.12,
September
15,
2014
ABSTRACT: Biofouling,
the accumulation of microorganisms, is a major problem in paper mills
processing paper and cardboard. This leads to the production of lower quality
recycled products. Several studies have focused on the microbial content in the
paper mill and the final products. Our aim was to determine the microbial biota
in a bale of collected cardboard prior to entering the paper mill. Total
genomic DNA was isolated and analyzed using two different methods for
comparison purposes: 454 pyrosequencing and clone library. A total of 3268
V6-V8 454 pyrosequencing reads and 322 cloned V6-V8 16S rRNA nucleotide
sequences were obtained. Both methods showed the presence of three major bacterial
genera: Bacillus, Solibacillus and Paenibacillus, all members of the spore-forming phylum Firmicutes.
Pyrosequencing, however, revealed a richer and more diverse bacterial community
than clone library. It showed the presence of additional minor Firmicute genera
and of a small number of Proteobacteria. The sorting at the recycling plant,
the storing, and the processing at the paper mill, the end uses, will all
contribute to the bacterial microbiota present in a bale of collected cardboard
as revealed here.