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Comparison of the Bacterial Microbiota in a Bale of Collected Cardboard Determined by 454 Pyrosequencing and Clone Library

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DOI: 10.4236/aim.2014.412082    2,655 Downloads   3,070 Views  

ABSTRACT

Biofouling, the accumulation of microorganisms, is a major problem in paper mills processing paper and cardboard. This leads to the production of lower quality recycled products. Several studies have focused on the microbial content in the paper mill and the final products. Our aim was to determine the microbial biota in a bale of collected cardboard prior to entering the paper mill. Total genomic DNA was isolated and analyzed using two different methods for comparison purposes: 454 pyrosequencing and clone library. A total of 3268 V6-V8 454 pyrosequencing reads and 322 cloned V6-V8 16S rRNA nucleotide sequences were obtained. Both methods showed the presence of three major bacterial genera: Bacillus, Solibacillus and Paenibacillus, all members of the spore-forming phylum Firmicutes. Pyrosequencing, however, revealed a richer and more diverse bacterial community than clone library. It showed the presence of additional minor Firmicute genera and of a small number of Proteobacteria. The sorting at the recycling plant, the storing, and the processing at the paper mill, the end uses, will all contribute to the bacterial microbiota present in a bale of collected cardboard as revealed here.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Lalande, V. , Barnabé, S. and Côté, J. (2014) Comparison of the Bacterial Microbiota in a Bale of Collected Cardboard Determined by 454 Pyrosequencing and Clone Library. Advances in Microbiology, 4, 754-760. doi: 10.4236/aim.2014.412082.

References

[1] Sorrelle, P.H. and Belgard, W.E. (1991) The Effect Of Recycled Fiber Use on Paper Machine Biological Control. TAPPI Proceedings, 569-575.
[2] Blanco, M.A., Negro, C., Gaspar, I. and Tijero, J. (1996) Slime Problems in Paper and Board Industry. Applied Microbiology and Biotechnology, 46, 203-220. http://dx.doi.org/10.1007/s002530050806
[3] Flemming, H.C., Meier, M. and Schild, T. (2013) Mini-Review: Microbial Problems in Paper Production. Biofouling, 29, 683-696. http://dx.doi.org/10.1080/08927014.2013.798865
[4] Milferstedt, K., Godon, J.J., Escudié, R., Prasse, S., Neyret, C. and Bernet, N. (2012) Heterogeneity and Spatial Distribution of Bacterial Background Contamination in Pulp and Process Water of a Paper Mill. Journal of Industrial Microbiology and Biotechnology, 39, 1751-1759.
http://dx.doi.org/10.1007/s10295-012-1196-8
[5] Vaisanen, O.M., Weber, A., Bennasar, A., Rainey, F.A., Busse, H.J. and Salkinoja-Salonen, M.S. (1998) Microbial Communities of Printing Paper Machines. Journal of Applied Microbiology, 84, 1069-1084. http://dx.doi.org/10.1046/j.1365-2672.1998.00447.x
[6] Desjardins, E. and Beaulieu, C. (2003) Identification of Bacteria Contaminating Pulp and a Paper Machine in a Canadian Paper Mill. Journal of Industrial Microbiology and Biotechnology, 30, 141-145.
[7] Suihko, M.L. and Skytta, E. (2009) Characterisation of Aerobically Grown Non-Spore-Forming Bacteria from Paper Mill Pulps Containing Recycled Fibres. Journal of Industrial Microbiology Biotechnology, 36, 53-64. http://dx.doi.org/10.1007/s10295-008-0472-0
[8] Oqvist, C.K., Kurola, J., Pakarinen, J., Ekman, J., Ikavalko, S., Simell, J. and Salkinoja-Salonen, M. (2008) Prokaryotic Microbiota of Recycled Paper Mills with Low or Zero Effluent. Journal of Industrial Microbiology and Biotechnology, 35, 1165-1173. http://dx.doi.org/10.1007/s10295-008-0396-8
[9] Pellegrin, V., Juretschko, S., Wagner, M. and Cottenceau, G. (1999) Morphological and Biochemical Properties of a Sphaerotilus sp. Isolated from Paper Mill Slimes. Appliedand Environmental Microbiology, 65, 156-162.
[10] Oppong, D., King, V.M., Zhou, X. and Bowen, J.A. (2000) Cultural and Biochemical Diversity of Pink-Pigmented Bacteria Isolated from Paper Mill Slimes. Journal of Industrial Microbiology and Biotechnology, 25, 74-80. http://dx.doi.org/10.1038/sj.jim.7000036
[11] Gendron, L.M., Trudel, L., Moineau, S. and Duchaine, C. (2012) Evaluation of Bacterial Contaminants Found on Unused Paper Towels and Possible Postcontamination after Handwashing: A Pilot Study. American Journal of Infection Control, 40, e5-e9. http://dx.doi.org/10.1016/j.ajic.2011.07.007
[12] Vaisanen, O.M., Nurmiaho-Lassila, E.L., Marmo, S.A. and Salkinoja-Salonen, M.S. (1994) Structure and Composition of Biological Slimes on Paper and Board Machines. Applied and Environmental Microbiology, 60, 641-653.
[13] Lahtinen, T., Kosonen, M., Tiirola, M., Vuento, M. and Oker-Blom, C. (2006) Diversity of Bacteria Contaminating Paper Machines. Journal of Industrial Microbiology and Biotechnology, 33, 734-740. http://dx.doi.org/10.1007/s10295-006-0105-4
[14] Granhall, U., Welsh, A., Throback, I.N., Hjort, K., Hansson, M. and Hallin, S. (2010) Bacterial Community Diversity in Paper Mills Processing Recycled Paper. Journal of Industrial Microbiology and Biotechnology, 37, 1061-1069. http://dx.doi.org/10.1007/s10295-010-0754-1
[15] Tiirola, M., Lahtinen, T., Vuento, M. and Oker-Blom, C. (2009) Early Succession of Bacterial Biofilms in Paper Machines. Journal of Industrial Microbiology and Biotechnology, 36, 929-937.
http://dx.doi.org/10.1007/s10295-009-0571-6
[16] Woese, C.R. (1987) Bacterial Evolution. Microbiological Reviews, 51, 221-271.
[17] Hugenholz, P., Goebel, B.M. and Pace, N.R. (1998) Impact of Culture-Independent Studies on the Emerging Phylogenetic View of Bacterial Diversity. Journal of Bacteriology, 180, 4765-4774.
[18] Sogin, M.L., Morrison, H.G., Huber, J.A., Welch, D.M., Huse, S.M., Neal, P.R., Arrieta, J.M. and Herndl, G.J. (2006) Microbial Diversity in the Deep Sea and the Underexplored “Rare Biosphere”. Proceedings of the National Academy of Sciences of the United States of America, 103, 12115-12120. http://dx.doi.org/10.1073/pnas.0605127103
[19] Comeau, A.M., Li, W.K.W., Tremblay, J.é., Carmack, E.C. and Lovejoy, C. (2011) Arctic Ocean Microbial Community Structure before and after the 2007 Record Sea Ice Minimum. PLoS ONE, 6, Article ID: e27492. http://dx.doi.org/10.1371/journal.pone.0027492
[20] Sanger, F., Nicklen, S. and Coulsonn, A.R. (1977) DNA Sequencing with Chain-Terminating Inhibitors. Proceedings of the National Academy of Sciences of the United States of America, 74, 5463-5467.
[21] Schloss, P.D. (2009) A High-Throughput DNA Sequence Aligner for Microbial Ecology Studies. PLoS ONE, 4, Article ID: e8230. http://dx.doi.org/10.1371/journal.pone.0008230
[22] Schloss, P.D., Westcott, S.L., Ryabin, T., Hall, J.R., Hartmann, M., Hollister, E.B., Lesniewski, R.A., Oakley, B.B., Parks, D.H., Robinson, C.J., Sahl, J.W., Stres, B., Thallinger, G.G., Van Horn, D.J. and Weber, C.F. (2009) Introducing Mothur: Open Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities. Applied and Environmental Microbiology, 75, 7537-7541. http://dx.doi.org/10.1128/AEM.01541-09
[23] Comeau, A.M., Harding, T., Galand, P.E., Vincent, W.F. and Lovejoy, C. (2012) Vertical Distribution of Microbial Communities in a Perennially Stratified Arctic Lake with Saline, Anoxic Bottom Waters. Scientific Reports, 2, Article No. 604. http://dx.doi.org/10.1038/srep00604
[24] Quast, C., Pruesse, E., Yilmaz, P., Gerken, J., Schweer, T., Yarza, P., Peplies, J. and Glockner, F.O. (2013) The SILVA Ribosomal RNA Gene Database Project: Improved Data Processing and Web-Based Tools. Nucleic Acids Research, 41, D590-D596. http://dx.doi.org/10.1093/nar/gks1219
[25] DeSantis, T.Z., Hugenholtz, P., Larsen, N., Rojas, M., Brodie, E.L., Keller, K., Huber, T., Dalevi, D., Hu, P. and Andersen, G.L. (2006) Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB. Applied and Environmental Microbiology, 72, 5069-5072.
http://dx.doi.org/10.1128/AEM.03006-05
[26] Werner, J.J., Koren, O., Hugenholtz, P., DeSantis, T.Z., Walters, W.A., Caposaro, J.G., Angenent, L.T., Knight, R. and Ley, R.E. (2012) Impact of Training Sets on Classification of High-Throughput Bacterial 16S rRNA Gene Surveys. The International Society for Microbial Ecology Journal, 6, 94-103.
[27] Disnard, J., Beaulieu, C. and Villemur, R. (2011) Composition of the Bacterial Biota in Slime Developed in Two Machines at a Canadian Paper Mill. Canadian Journal of Microbiology, 57, 91-104.
http://dx.doi.org/10.1139/W10-109
[28] Ratto, M., Suihko, M.L. and Siika-aho, M. (2005) Polysaccharide-Producing Bacteria Isolated from Paper Machine Slime Deposits. Journal of Industrial Microbiology and Biotechnology, 25, 109-114. http://dx.doi.org/10.1007/s10295-005-0210-9
[29] Suihko, M.L. and Stackebrandt, E. (2003) Identification of Aerobic Mesophilic Bacilli Isolated from Board and Paper Products Containing Products Containing Recycled Fibres. Journal of Applied Microbiology, 94, 25-34. http://dx.doi.org/10.1046/j.1365-2672.2003.01803.x
[30] Suominen, I., Suihko, M.L. and Salkinoja-Salonen, M. (1997) Microscopic Study of Migration of Microbes in Food-Packaging Paper and Board. Journal of Industrial Microbiology and Biotechnology, 19, 104-113. http://dx.doi.org/10.1038/sj.jim.2900424
[31] Vaisanen, O.M., Mentu, J. and Salkinoja-Salonen, M. (1991) Bacteria in Food Packaging Paper and Board. Journal of Applied Bacteriology, 71, 130-133.
http://dx.doi.org/10.1111/j.1365-2672.1991.tb02967.x
[32] Krishnamurthi, S., Chakrabarti, T. and Stackebrandt, E. (2009) Re-Examination of the Taxonomic Position of Bacillus silvestris Rheims et al. 1999 and Proposal to Transfer It to Solibacillus gen. nov. as Solibacillus silvestris comb. nov. International Journal of Systematic and Evolutionary Microbiology, 59, 1054-1058.
[33] Namjoshi, K., Johnson, S., Montello, P. and Pullman, G.S. (2010) Survey of Bacterial Populations Present in US-Produced Linerboard with High Recycle Content. Journal of Applied Microbiology, 108, 416-427. http://dx.doi.org/10.1111/j.1365-2672.2009.04437.x

  
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