Share This Article:

DNA Variation of Capoeta damascina (Valenciennes, 1842) in Three Rivers in Northern Israel

Abstract Full-Text HTML Download Download as PDF (Size:1547KB) PP. 107-117
DOI: 10.4236/jbpc.2014.53012    3,311 Downloads   3,904 Views  
Author(s)    Leave a comment

ABSTRACT

The present study is in agreement with the hypothesis that the variation of ecological conditions in three rivers in northern Israel—the Dan, Hasbani and Hermon Rivers—affects the genetic variations of the species Capoeta damascina. Using mitochondrial DNA (mtDNA), cytochrome b gene (Cytb), 16S and nuclear DNA (nDNA), and Random Amplified Polymorphic DNA (RAPD), four different clusters were found in the Cytb of the Hasbani and Hermon Rivers and only two in the Dan River. Moreover, the clusters in the Hasbani River differed from those found in the Hermon River. A similar result was found when an analysis was made of a different sequence from five different haplotype frequencies using the MegAlign program, the lowest being in the Dan River (only two haplotypes) and the highest in the Hasbani River (four haplotypes). The analysis of molecular variance of Cytb and 16S (AMOVA) for individuals of C. damascina from eight populations in northern Israel showed significant differences between the rivers and the populations. The analysis by mitochondrial 16S of haplotype frequencies of C. damascina populations in the rivers in northern Israel was very low compared to Ctb. Sixteen different haplotypes were found in the different rivers: eight in the Hasbani River, seven in the Dan River and only five in the Hermon River.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Degani, G. (2014) DNA Variation of Capoeta damascina (Valenciennes, 1842) in Three Rivers in Northern Israel. Journal of Biophysical Chemistry, 5, 107-117. doi: 10.4236/jbpc.2014.53012.

References

[1] Levin, B.A., Freyhof, J., Lajbner, Z., Perea, S., Abdoli, A., Gaffaroglu, M., Ozulug, M., Rubenyan, H.R., Salnikov, V.B. and Doadrio, I. (2012) Phylogenetic Relationships of the Algae Scraping Cyprinid Genus Capoeta (Teleostei: Cyprinidae). Molecular Phylogenetics and Evolution, 62, 542-549.
[2] Betts, A.M., Stone, D.M., Way, K., Torhy, C., Chilmonczyk, S., Benmansour, A. and de Kinkelin, P. (2003) Emerging Vesiculo-Type Virus Infections of Freshwater Fishes in Europe. Diseases of Aquatic Organisms, 57, 201-212.
[3] Durand, J.D., Tsigenopoulos, C.S., Unlu, E. and Berrebi, P. (2002) Phylogeny and Biogeography of the Family Cyprinidae in the Middle East Inferred from Cytochrome b DNA—Evolutionary Significance of This Region. Molecular Phylogenetics and Evolution, 22, 91-100.
[4] Degani, G., Herbst, G.N., Berman, T., Netzer, Y. and Ortal, R. (1989) Physical and Chemical Factors in the Dan River and Their Relationship to Algae Colonization Rates. Physical and Chemical Factors in the Dan River and Their Relationship to Algae Colonization Rates. 4th International Conference: Environment Quality and Ecosystem Stability, 359-368.
[5] Degani, G., Herbst, G.N., Ortal, R., Bromley, H.J., Levanon, D., Glazman, H. and Regev, Y. (1992) Faunal Relationships to Abiotic Factors along the River Dan in Northern Israel. Hydrobiologia, 246, 69-82.
[6] Degani, G., Herbst, G.N., Ortal, R., Bromley, H.J., Levanon, D., Netzer, Y., Harari, N. and Glazman, H. (1993) Relationship between Current Velocity, Depth and the Invertebrate Community in a Stable River System. Hydrobiologia, 263, 163-172.
[7] Amery, H. (2002) Water Wars in the Middle East: A Looming Threat. GeoJournal, 168, 1-11.
[8] Markova, S., Sanda, R., Crivelli, A., Shumka, S., Wilson, I.F., Vukic, J., Berrebi, P. and Kotlik, P. (2010) Nuclear and Mitochondrial DNA Sequence Data Reveal the Evolutionary History of Barbus (Cyprinidae) in the Ancient Lake Systems of the Balkans. Molecular Phylogenetics and Evolution, 55, 488-500.
[9] Bercovich, D., Korem, S., Shauder, L. and Degani, G. (2012) Genetic Diversity of Color Phenotypes in the Koi (Cyprinus carpio L.) as Identified by Molecular Markers. Biophysical Chemistry, 3, 249-255.
[10] Wilbur, W.J. and Lipman, D.J. (1983) Rapid Similarity Searches of Nucleic Acid and Protein Data Banks. Proceedings of the National Academy of Sciences of the United States of America, 80, 726-730.
[11] Peakall, R. and Smouse, P.E. (2006) GENALEX 6: Genetic Analysis in Excel. Population Genetic Software for Teaching and Research. Molecular Ecology Notes, 6, 288-295.
[12] Slatkin, M. (1985) Gene Flow in Natural Populations. Annual Review of Ecology, Evolution, and Systematics, 16, 393- 430.
[13] Nei, M. (1972) Genetic Distance between Populations. American Naturalist, 106, 283-292.
[14] Nei, M. (1978) Estimation of Average Heterozygosity and Genetic Distance from a Small Number of Individuals. Genetics, 89, 583-590.
[15] Saitou, N. and Nei, M. (1987) The Neighbor-Joining Method: A New Method for Reconstructing Phylogenetic Trees. Molecular Biology and Evolution, 4, 406-425.
[16] Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731-2739.
[17] Veness, C. (2011) Calculate Distance, Bearing and More between Latitude/Longitude Points. Movable Type Scripts. Information Design & Management, Welsh Prose, 1300-1425.
[18] Mantel, N. (1967) The Detection of Disease Clustering and a Generalized Regression Approach. Cancer Research, 27, 209-220.
[19] Corander, J., Marttinen, P., Sirén, J. and Tang, J. (2006) BAPS v.4.14: Bayesian Analysis of Population Structure.
http://www.rni.helsinki.fi/~jic/bapspage.html
[20] Excoffier, L., Smouse, P.E. and Quattro, J.M. (1992) Analysis of Molecular Variance Inferred from Metric Distances among DNA Haplotypes: Application to Human Mitochondrial DNA Restriction Data. Genetics, 131, 479-491.
[21] Goren, M. and Ortal, R. (1999) Biogeography, Diversity and Conservation of the Inland Water Fish Communities in Israel. Biological Conservation, 89, 1-9.
[22] Degani, G., Goldberg, T., Gazith, A., Elorom, E. and Nevo, E. (2013) The DNA Variation of Pseudepidalea viridis (Syn. Bufo viridis) from Various Habitats. Zoological Studies, 52, 1-15.
[23] Degani, G., Nagar, R. and Yom-Din, S. (2012) Molecular DNA Variation in Hyla felixarabica, Herpetol. Romanica, 6 51-67.
[24] Degani, G. and Elad Biton, B. (2013) Tree Frog (Hyla savygnyi) Color and Substrate Preference. American Open Animal Science Journal, 3, 31-39.
[25] Pearlson, O., Blaustein, L., Goldberg, D. and Degani, D. (2005) Molecular DNA Variation and Mitochondrial Sequence Analysis of Triturus vittatus vittatus (Urodela) from Breeding Sites at Various Altitudes in the Southern Limit of Its Distribution. FISEB Federation of the Israel Societies for Experimental Biology, Eilat.
[26] Pearlson, O. and Degani, G. (2007) Triturus v. vittatus (Urodela) Larvae at Various Breeding Sites in Israel. Progrese si Perspective in Medicina Veterinara, 50, 214-226.
[27] Pearlson, O. and Degani, G. (2007) Molecular DNA Variations among Triturus vittatus vittatus (Urodela) from Different Breeding Sites at the Southern Limit of Its Distribution. Acta Herpetological Journal, 2, 69-77.
[28] Pearlson, O. and Degani, G. (2007) Triturus v. vittatus (Urodela) Larvae at Various Breeding Sites in Israel. Progrese si Perspective in Medicina Veterinara, 50, 214-226.
[29] Pearlson, O., Bluaustin, L., Snir, S., Goldberg, G. and Degani, G. (2010) Molecular DNA Variation and Mitochondrial Sequence Analysis of Triturus vittatus vittatus (Urodela) from Various Breeding Sites near the Southern Limit of Its Distribution. Current Herpetology, 29, 11-22.
[30] Degani, G. (2013) Mitochondrial DNA Sequence Analysis in Anabantoidei Fish. Advances in Biological Chemistry, 3, 347-355.
[31] Goldberg, T., Pearlson, O., Nevo, E. and Degani, G. (2007) Mitochondrial DNA Analysis of Salamandra infraimmaculata Larvae from Habitats in Northern Israel. Progrese si Perspective in Medicina Veterinara-Lucrari Stiintifice, 50, 23-31.
[32] Goldberg, T., Eviatar, E. and Degani, G. (2009) Sequence Analysis of Mitochondrial DNA in Salamandra infraimmaculata Larvae from Populations in Northern Israel. South American Journal of Herpetology, 4, 268-274.
[33] Goldberg, T., Nevo, E. and Degani, G. (2011) Genetic Diverseness and Different Ecological Condition in Salamnadra infraimaculata Larvae from Various Breeding Places. Animal Biology, 2, 37-49.
[34] Degani, G., Goldberg, T. and Nevo, E. (2014) Genetic Variation in Salamandra infraimmaculata from Different Habitats Using Amplified Fragment Length Olymorphism. Biophysical Chemistry, 5, 54-66.
[35] Ayatollah, S., Soofiani, L.N.M., Keivany, Y. and Shadkhast, M. (2011) Reproduction of Capoeta damascina (Valenciennes, 1842), a Cyprinid Fish in Zayandeh-Roud River, Iran. Journal of Applied Ichthyology, 27, 1061-1066.

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.