Author(s)

Ahmed El-Sayed Nour El-Deen^1*, Abd El-Megeed Mansour¹, Ahmad Taha², Ehab M. Fahmy³

¹Department of Physiology, Faculty of Medicine, Al-Azhar University, Assuit, Egypt.
²Department of Physiology, Faculty of Medicine, Port Said University, Port Said, Egypt.
³Department of Medical Microbiology and Immunology, Faculty of Medicine, Aswan University, Aswan, Egypt.

Background: Green coffee as a functional food has an antioxidant effect, which can reduce the cancer incidence, promote weight-loss and improve glucose and lipid metabolism, as well as anti-oxidant and anti-inflammatory activity. Objective: Assessing the effect of green coffee administration on experimental cisplatin induced renal apoptosis in adult male albino rats of local strain. Design: Randomized Block Design was used. Materials and Methods: Forty adult male albino rats of local strain were randomly divided into four groups of 10 animals each. G1: control negative group was received isotonic saline (0.5 ml, i.p.) for 30 consecutive days, G2: sham operated group mice were received green coffee extract in water (40 mg/kg/day) for 30 consecutive days, animals of G3 and G4 were administered cisplatin (8 mg/kg/day, i.p.) at 10th day, which is well known to produce significant nephrotoxicity in rats. Animals in G3 were received green coffee extract in water (40 mg/kg/day) and G4 was received isotonic normal saline (0.5 ml, i.p.) for 30 consecutive days. All rats were sacrificed after 30 days and blood was withdrawn for biochemical examinations of kidney function tests (blood urea nitrogen, creatinine and uric acid). Kidneys were removed for determination of renal oxidative stress markers (H₂O₂) biochemically and caspase-3 by Immunohistochemical examination. Results: Cisplatin administration was associated with significant higher levels of BUN, creatinine, uric acid and H₂O₂ as compared with normal control group. Green coffee administration in cisplatin-induced renal apoptosis groups produced significant lower levels of BUN, creatinine, uric acid and H₂O₂ (24.4 ± 4.14, 1.730 ± 0.2830, 5.50 ± 0.850 and 0.51 ± 0.12 respectively) as compared with cisplatin-induced renal apoptosis group not administrated green coffee (27.4 ± 6, 2.04 ± .31, 7.00 ± 1.25 and 1.1 ± 0.16 respectively). Cisplatin administration increased expression of the apoptotic protein caspase-3. In contrast, treatment with green coffee extract attenuated apoptosis. Conclusion: green coffee reduced cisplatin-induced renal apoptosis. Green coffee improved the general condition of cisplatin-induced renal apoptosis rats due to its antioxidant and anti-apoptotic effects.

Green Coffee, Cisplatin and Apoptosis

El-Deen, A. , Mansour, A. , Taha, A. and Fahmy, E. (2019) Effect of Green Coffee on Cisplatin Induced Renal Apoptosis in Adult Male Albino Rats. Food and Nutrition Sciences, 10, 358-368. doi: 10.4236/fns.2019.104028.