Author(s)

Asmaa Meraou^1,2, Hafida Merzouk¹, Amel Saidi¹, Amel Medjdoub¹, Sid Ahmed Merzouk³, Slimane Belbraouet^4*

¹Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University Abou-Bekr Belkaid of Tlemcen, Tlemcen, Algeria.
²University Djilali liabes of Sidi Bel Abbes, Sidi Bel Abbes, Algeria.
³Department of Technical Sciences, Faculty of Engineering, University Abou-Bekr Belkaid of Tlemcen, Tlemcen, Algeria.
⁴Ecole de Nutrition, Université de Moncton, Moncton, Canada.

Background: The prevalence of overweight and obesity associated with oxidative stress and immune abnormalities is continuously increasing. Antioxidant supplementations might counteract potential damage caused by ROS to cellular tissues. Objective: To determine the role of vitamins on immune improvement during obesity, we investigated in vitro effects of vitamins C, E, and NADH on mitogen-stimulated proliferation, Th1- and Th2-type cytokine production, and oxidant/antioxidant status of lymphocytes isolated from obese patients. Methods: Peripheral blood lymphocytes were isolated using a density gradient of Histopaque. They were in vitro cultured and stimulated by Con A in the presence or absence of vitamins. Cell proliferation was determined by MTT assay and interleukin-2, interleukin-4 and interferon-γ (INFγ) secretions. Cell oxidant/antioxidant balance was studied by assaying glutathione (GSH), malondialdehyde (MDA), carbonyl protein levels, catalase activity and micronucli frequency. Results: Obesity is associated with enhanced oxidative stress response. Indeed, vitamin C, E and NADH improved significantly lymphocyte proliferation and diminished cellular oxidative stress. Conclusion: Treatments of lymphocytes with vitamins had beneficial effects on lymphocyte proliferation, cytokines secretions and redox status, generating an anti-inflammatory profile and should be considered in therapeutic approaches for normalizing immune cell function in obesity.

Obesity, Lymphocytes, Vitamins, Cytokines, Oxidant/Antioxidant Status

Meraou, A. , Merzouk, H. , Saidi, A. , Medjdoub, A. , Merzouk, S. and Belbraouet, S. (2016) Vitamins C, E, and NADH on in Vitro Lymphocyte Proliferation and Redox Status among Obese Patients. Food and Nutrition Sciences, 7, 1082-1098. doi: 10.4236/fns.2016.712104.