Suitability of the Composite Made of Multi Wall Carbon Nanotubes-Polyvinylpyrrolidone for Culturing Invertebrate Helix aspersa Neurons

Full-Text HTML XML Download Download as PDF (Size:663KB) PP. 41-50
DOI: 10.4236/msce.2017.52005    743 Downloads   895 Views  


Carbon nanotubes have been used as scaffolds for tissue engineering. However, the identification of these nanomaterials disperses in biological solutions and their direct interaction with nerve cells is still controversial. The aim of this work is to characterize the topographic and ultra-structural distribution of the composite made of multi wall carbon nanotubes-polyvinylpyrrolidone (MWCNTs-PVP) deposited on the Helix aspersa neurons and over glass coverslip. Scanning Electron Microscopy (SEM) and Confocal Microscopy (CM) studies were done to analyze the properties of such MWCNTs-PVP composite. The cerebral ganglion of Helix aspersa was treated and incubated with MWCNTs-PVP, fixing it in paraformaldehyde at 4% and was observed with SEM and CM. Although the nanotubes were not labeled or stained with fluorescent compounds, the MWCNTs-PVP deposited on glass and on nerve cells, was observed by the confocal microscope in the reflection mode. In SEM studies, it was observed that MWCNTs-PVP was attached to the surface on neurons. Moreover, in CM studies, it was possible to observe that MWCNTs-PVP was attached to the neuronal membrane, crossing the cell membrane and getting into the cytoplasm. These results support the hypothesis that carbon nanotubes interact with the neuronal cell membrane and can be useful for neuronal tissue engineering. In addition, these results open new alternatives for toxicological studies, in order to elucidate the cytotoxicity of MWCNTs-PVP composite in neurons and other excitable cells.

Cite this paper

Bernal-Martinez, J. , Godínez-Fernández, R. and Aguilar-Elguezabal, A. (2017) Suitability of the Composite Made of Multi Wall Carbon Nanotubes-Polyvinylpyrrolidone for Culturing Invertebrate Helix aspersa Neurons. Journal of Materials Science and Chemical Engineering, 5, 41-50. doi: 10.4236/msce.2017.52005.


[1] Harrison, B.S. and Atala, A. (2007) Carbon Nanotube Applications for Tissue Engineering. Biomaterials, 28, 344-353.
[2] Abarrategia, A., Gutiérrez, M.C., Moreno-Vicente, C., Hortigüel, M.J., Ramos, V., López-Lacomba, J.L., Ferrer, M.L. and del Monte, F. (2008) Multiwall Carbon Nanotube Scaffolds for Tissue Engineering Purposes. Biomaterials, 29, 94-102.
[3] Haniu, H., et al. (2012) Basic Potential of Carbon Nanotubes in Tissue Engineering Applications. Journal of Nanomaterials, 2012, Article ID: 343747.
[4] Kim, J.S., Song, K.S. and Yu, I.J. (2016) Multiwall Carbon Nanotube-Induced DNA Damage and Cytotoxicity in Male Human Peripheral Blood Lymphocytes. International Journal of Toxicology, 35, 27-37.
[5] Montes-Fonseca, S.L., Orrantia-Borunda, E., Aguilar-Elguezabal, A., González Horta, C., Talamás-Rohana, P. and Sánchez-Ramírez, B. (2012) Cytotoxicity of Functionalized Carbonnanotubes in J774A Macrophages. Nanomedicine, 8, 853-859.
[6] Sachar, S. and Saxena, R.K. (2011) Cytotoxic Effect of Poly-Dispersed Single Walled Carbon Nanotubes on Erythrocytes in Vitro and in Vivo. PLoS ONE, 6, e22032.
[7] Fabbro, A., Prato, M. and Ballerini, L. (2013) Carbon Nanotubes in Neuroregeneration and Repair. Advanced Drug Delivery Reviews, 65, 2034-2044.
[8] Fabbro, A., Sucapane, A., Toma, F.M., Calura, E., Rizzetto, L., Carrieri, C., Roncaglia, P., Martinelli, V., Scaini, D., Masten, L., Turco, A., Gustincich, S., Prato, M. and Ballerini, L. (2013) Adhesion to Carbon Nanotube Conductive Scaffolds Forces Action-Potential Appearance in Immature Rat Spinal Neurons. PLoS ONE, 8, e73621.
[9] Martinelli, V., Cellot, G., Fabbro, A., Bosi, S., Mestroni, L. and Ballerini, L. (2013) Improving Cardiac Myocytes Performance by Carbon Nanotubes Platforms. Frontiers in Physiology, 4, 239.
[10] Bosi, S., Rauti, R., Laishram, J., Turco, A., Lonardoni, D., Prato, M., Scaini, D. and Ballerini, L. (2015) From 2D to 3D: Novel Nanostructured Scaffolds to Investigate Signalling in Reconstructed Neuronal Networks. Scientific Reports, 5, 9562.
[11] Hirsch, A. (2002) Functionalization of Single-Walled Carbon Nanotubes. Angewandte Chemie International Edition, 41, 1853-1859.<1853::AID-ANIE1853>3.0.CO;2-N
[12] Achaby, M.E., Arrakhiz, F.E., Vaudreuil, S., Essassi, M., Qaiss, M. and Bousmina, M. (2013) Nanocomposite Films of Poly(Vinylidene Fluoride) Filled with Polyviny-lpyrrolidone-Coated Multiwalled Carbon Nanotubes: Enhancement of b-Polymorph Formation and Tensile Properties. Polymer Engineering and Science, 53, 34-43.
[13] Nasouri, K., Shoushtari, A.M. and Mojtahedi, M.R.M. (2016) Synthesis and Characterization of Highly Dispersed Multi-Walled Carbon Nanotubes/Polyvinyl-Pyrrolidone Composite Nanofibers for EMI Shielding Application. Polymer Composites.
[14] Bernal-Martínez, J., Seseña-Rubfiaro, A., Godínez-Fernández, R. and Aguilar-Elguezabal, A. (2016) Electrodes Made of Multi-Wall Carbon Nanotubes on PVDF-Filters Have Low Electrical Resistance and Are Able to Record Electrocardiograms in Humans. Microelectronic Engineering, 166, 10-14.
[15] Bernal-Martínez, J., Godínez-Fernández, R., Roman-Aguirre, M. and Aguilar-El-guezabal, A. (2016) The Electrical Resistance of Electrodes Made of Multi Walled Carbon Nanotubes Is Modulated by nIR-Laser. Microelectronic Engineering, 166, 45-49.
[16] Shityakov, S., Salvador, E., Pastorin, G. and Förster, C. (2015) Blood-Brain Barrier Transport, Aggregation, and Molecular Dynamics Simulation of Multiwalled Carbon Nanotube Functionalized with Fluorescein Isothiocyanate. International Journal of Nanomedicine, 10, 1703-1713.
[17] Iancu, C., Mocan, L., Bele, C., Orza, A.I., Tabaran, F.A., Catoi, C., Stiufiuc, R., Stir, A., Matea, C., Iancu, D., Agoston-Coldea, L., Zaharie, F. and Mocan, T. (2011) Enhanced Laser Thermal Ablation for the in Vitro Treatment of Liver Cancer by Specific Delivery of Multiwalled Carbon Nanotubes Functionalized with Human Serum Albumin. International Journal of Nanomedicine, 6, 129-141.
[18] Hao, Y., Yang, X., Shi, Y., Xing, J., Marowitch, J., Chen, J. and Chen, J. (2012) FITC Delivery into Plant Cells Using Magnetic Single-Walled Carbon Nanotubes. Journal of Nanoscience and Nanotechnology, 12, 6287-6293.
[19] Chen, M.T., Gomez, L.M., Ishikawa, F.N., Vernier, P.T., Zhou, C. and Gundersen, M.A. (2009) PH-Sensitive Intracellular Photoluminescence of Carbon Nanotube-Fluorescein Conjugates in Human Ovarian Cancer Cells. Nanotechnology, 20, Article ID: 295101.
[20] Aguilar-Elguezabal, A., Antunez, W., Alonso, G., Delgado, F.P., Espinosa, F. and Miki-Yoshida, M. (2006) Study of Carbon Nanotubes Synthesis by Spray Pyrolysis and Model of Growth. Diamond & Related Materials, 15, 1329-1335.
[21] Zaragoza-Contreras, E.A., Lozano-Rodríguez, E.D., Román-Aguirre, M., Antunez-Flores, W., Hernández-Escobar, C.A., Flores-Gallardo, S.G. and Aguilar-Elgue-za-bal, A. (2009) Evidence of Multi-Walled Carbon Nanotube Fragmentation Induced by Sonication during Nanotube Encapsulation via Bulk-Suspension Polymerization. Micron, 40, 621-627.
[22] Kerkut, G.A., et al. (1973) Mapping of Nerve Cells in the Suboesophageal Ganglia of Helix Aspersa. Comparative Biochemistry and Physiology, 50, 1-25.
[23] Schindelin, J., et al. (2012) Fiji: An Open-Source Platform for Biological-Image Analysis. Nature Methods, 9, 676-682.
[24] Tan, X.Q., Cheng, X.L., Zhang, L., Wu, B.W., Liu, Q.H., Meng, J., Xu, H.Y. and Cao, J.M. (2014) Multi-Walled Carbon Nanotubes Impair Kv4.2/4.3 Channel Activities, Delay Membrane Repolarization and Induce Bradyarrhythmias in the Rat. PLoS ONE, 9, e101545.
[25] Chen, T., Yang, J., Ren, G., Yang, Z. and Zhang, T. (2013) Multi-Walled Carbon Nanotube Increases the Excitability of Hippocampal CA1 Neurons through Inhibition of Potassium Channels in Rat’s Brain Slices. Toxicology Letters, 217, 121-128.
[26] Xu, H., Bai, J., Meng, J., Hao, W., Xu, H. and Cao, J.M. (2009) Multi-Walled Carbon Nanotubes Suppress Potassium Channel Activities in PC12 Cells. Nanotechnology, 20, Article ID: 285102.

comments powered by Disqus

Copyright © 2017 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.