Tyrosine phosphorylation of monocyte-derived macrophage proteins in buffalo (Bubalus bubalis): A potential phenotype of natural resistance

Maria Miarelli, Federica Signorelli, Giovanna de Matteis

Consiglio per la Ricerca e la Sperimentazione in Agricoltura (Agricultural Research Council), Monterotondo, Italy.

DOI: 10.4236/ojas.2013.32019                

Abstract

The aim of this work was to explore the possibility of using the presence of tyrosine-phosphorylated macrophage proteins as a phenotype of natural resistance. Tyrosine-phosphorylation of macrophage proteins was investigated in 18 buffaloes, that carried either the resistant, or the non-resistant, Natural Resistance-Associated Macrophage Protein one (NRAMP1) genotype, that various authors have associated with susceptibility to intracellular bacterial diseases. Monocyte-derived macrophages were Interferon-gamma (IFN-γ) stimulated and tyrosine-phosphorylation was assessed by Western blotting. Evidence of phosphorylation after IFN-γ stimulation was shown by 75% of the buffaloes carriers of the resistant genotype, and by 20% of the carriers of the non-resistant genotype (Chisquare value between the groups = 5.44; P = 0.02). The study of the Proteoma of monocyte-derived macrophages might open the way to the genetic control of disease resistance.

Keywords

Buffalo; Macrophage; Natural Resistance; Tyrosine Phosphorylation

Conflicts of Interest

The authors declare no conflicts of interest.

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