USEPA, “Method 1603: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified Membrane-Thermotolerant Escherichia coli agar (Modified m TEC),” Publication EPA-821-R-02-023, USEPA Office of Water, Office of Science and Technology, Washington DC, 2002.

has been cited by the following article:

• TITLE: Growth Kinetics of Wildlife E. coli Isolates in Soil and Water

  AUTHORS: Meghan A. Gallagher, Raghupathy Karthikeyan, Saqib Mukhtar

  KEYWORDS: Bacteria; Fecal Contamination; TMDL; Water Quality

  JOURNAL NAME: Journal of Environmental Protection, Vol.3 No.8A, August 21, 2012

  ABSTRACT: Bacteria are the major cause of surface water contamination in the United States. US Environmental Protection Agency (USEPA) uses the Total Maximum Daily Load (TMDL) process to regulate the E. coli loads from fecal sources in a watershed. Different point and non-point sources can contribute to the fecal contamination of a waterbody including municipal and on-site wastewater treatment plants, livestock, birds, and wildlife. Unfortunately, wildlife sources in many rural watersheds are poorly characterized. E. coli is also known to persist in waterbodies when no known fecal sources are present. In this study, E. coli from wildlife fecal material was enumerated and the fate of E. coli under different environmental factors was studied. No growth was observed in soil at 4% moisture content and in water at 10℃. The highest E. coli growth was recorded in water at 30℃. It can be seen from these results that there was variation in the fate of E. coli under different environmental conditions. The fate of E. coli in the environment is a complex process and is influenced by many factors and their interactions, making it difficult to predict. The findings from this study along with additional studies can be used to improve the accuracy of model predictions to estimate the E. coli loads in watersheds.