An experiment was carried out for identification and determination of malathion degrading phosphate solubilizing bacteria isolated from the agricultural fields. In this study, malathion degrading phosphate solubilizing bacteria were identified using NBRIP (National Botanical Research Institute’s phosphate growth medium) media. A number of bacterial colonies were screened from agricultural fields. From primarily screened colonies 4 isolates were identified as phosphate solubilizing bacteria through qualitative and quantitative analysis. The isolated 4 bacterial colonies were inoculated in NBRIP broth media enriched with malathion pesticides to observe degradation of malathion pesticide under incubation study at three different temperatures (25°C, 30°C and 37°C). However, all the four isolates showed capability in degrading malathion pesticide. The study clearly revealed that phosphate solubilizing bacteria can be used in bioremediation of environmental pollution caused by malathion pesticide.
Bangladesh is facing many challenges in agricultural sector in the coming years. In order to secure food demand for its vast population, more toxic chemicals are used that have reputations of speed and effectiveness to increase food production. The Government of Bangladesh also promotes the use of pesticides to increase output per acre of land. Overall, pesticide use in general is increasing.
They provide unquestionable benefit for agricultural production, even though, as a consequence, low amounts of some residues may persist in the food supply, air, water and soil and could constitute a significant exposure pathway for humans (Ghorab & Khalil, 2016) . The growing use of pesticides in contemporary agriculture has direct or indirect impact on the composition of soil microorganisms. It is stated that usually more than 109 microorganisms are present per gram of soil representing 4000 to 7000 different genomes and biomass of 300 to 3000 kg per ha (Ranjard & Richaume, 2001) which directly or indirectly are affected by many conditions of these insecticides used. If pesticides can be degraded as soon as they are applied, then their desired function will not be served.
Pesticide biodegradation by microorganisms has been documented in a wide range of habitats, including soils, ground water and sewage sludges etc. Insecticides and their degradation products generally get accumulated in the top soil and influence not only the population of various groups of soil microbes but also their biochemical activities like nitrification, ammonification, decomposition of organic matter and nitrogen fixation (Agnihotri et al., 1981) . Recently, use of microbes for effective detoxifying, degrading and removal of toxic compounds from contaminated soil and water has emerged as an efficient technique to clean up polluted environments (Strong & Burgess, 2008) .
Malathion [diethyl 2-{(dimethoxyphosphorothioyl) sulfanyl} butanedioate] is one of the common pesticides used in pest control programs in Bangladesh. In this study malathion was degraded by using Phosphate solubilizing bacteria under laboratory condition. As it is reported that phosphate solubilizing bacteria are capable of mineralizing organic phosphorous compound, so it is expected that these bacteria will be able to degrade organophosphate malathion releasing soluble phosphorous.
So, the efficiency of the isolated microorganisms to metabolize malathion in order to develop safe, convenient and economically feasible methods for pesticide detoxification would be investigated under this study.
Soils were collected from the area of Sirajganj, Tangail and Madhupur (
Sirajganj: Soil sample was collected from agricultural field of Sirajganj. The GPS location of the sampling point where soil samples were collected was approximately 24˚24'27.7164''N and 89˚38'7.1376''E. Sirajganj falls under Precambrian platform and is characterized by sedimentary rocks above a Precambrian igneous and metamorphic basement. The area lies in the active delta region of
Brahmmaputra-Jamuna floodplain and general soil type is grey floodplain soils (
Tangail: Soil sample was collected from agricultural field which is situated beside Joydevpur-Tangail-Jamalpur highway. The GPS location of the sampling point where soil samples were collected was approximately 24˚19'35.1336''N and 89˚55'33.7656''E. The parent material of soil is Brahmaputra-Jamuna alluvium and general soil type is grey floodplain soils (
Gazipur: The sample is collected from agricultural site which is situated in Zirani bazar, Madhupur, Gazipur district. The GPS location of the sampling point where soil samples were collected was approximately 23˚59'45.5856''N and 90˚15'21.564''E. The area is categorized under red-brown general soil type (
Malathion (57%) a commercial grade pesticide used mainly for pest control was collected from local pesticide shop from Dhaka, Bangladesh. The pesticide belongs to organophosphate class of pesticides.
The samples were collected from 8 - 10 cm depth using a sterile spatula and transferred to autoclaved Ziplock bag stored under room temperature.
Oxidase test, Catalase test, Oxygen requirement, Gelatin liquefaction, indole production test, methyl red and Voges-Proskauer test, citrate utilization, starch hydrolysis, Deamination of Phenylalanine Acid Production from D-Glucose, Gas Production from D-Glucose, Nitrate reduction and Triple sugar iron test. Furthermore, quantitative and qualitative tests were carried out by using National Botanical Research Institute’s phosphate growth medium (NBRIP) to identify phosphate solubilizing bacteria. By measuring the amount of phosphorous solubilized and appearance of halo zones, the identified bacteria were considered as phosphate solubilizing bacteria (
For determining the efficiency of isolated microorganism in degrading malathion pesticide National Botanical Research Institute’s phosphate growth medium (NBRIP) broth (modified)-contained glucose, 10 g: malathion [instead of Ca3(PO4)2]; MgCl2・6H2O, 5 g; MgSO4・7H2O, 0.25 g; KCl, 0.2 g and (NH4)2SO4, 0.1 g was used. The freshly prepared broths were carried out using autoclavable falcon (50 ml) containing 20 ml of medium and autoclaved at 121˚C for 15 minutes and incubated at 30˚C overnight. In the next day, broths were inoculated with isolated phosphate solubilizing bacterial colony (200 μl inoculum with approximately 2 - 3 × 109 cfu∙ml−1) in triplicate. Autoclaved uninoculated medium served as control. The broths were incubated for 2, 4, 6 and 10 days at different temperature (25˚C, 30˚C, 37˚C). The cultures were harvested by centrifugation at 10,000 rpm for 10 min, using centrifugal machine (Hettich Zentrifugen, Germany). Then the samples were filtered for phosphate determination (
All soil conditions may not be suitable for growing all types of microorganisms. Vikram et al. (2007) reported a strong positive correlation between organic carbon content, available N and PSB population. Also, Ndung’u-Magiroi et al. (2012) showed a positive and highly significant correlation between PSB and phosphate-solubilizing microorganism (PSM) populations, organic C, exchangeable Ca and exchangeable Mg, while pH and extractable P did not correlate with the PSB population. Winogradsky detected saprophytic Bacillus species in soil, stating that the bacteria’s spores became active when an excess of easily decomposable organic matter became available or when the moisture content of the soil was high (Winogradsky, 1924) . Pseudomonas aeruginosa is found widely in nature, in soil and water and has few nutritional requirements and can adapt to conditions not tolerated by other organisms (Prince, 2012) . Therefore, the chemical parameters of soil samples were measured to determine favorable condition for the phosphate solubilizing Bactteria (
In the present experiment, malathion was the sole phosphorous source for microbes in the broth. The amount of soluble phosphate released in the medium proved the degradation of malathion. Malathion degradation was observed at 25˚C, 30˚C and 37˚C which were presented in Tables 3-5. The phosphorous releasing capacity of these microbes varied with timings and incubation temperature.
Parameter | Soil 1 | Soil 2 | Soil 3 |
---|---|---|---|
Moisture content (%) | 9.66 | 17.21 | 12.37 |
pH | 7.85 | 6.90 | 4.25 |
Total Carbon (%) | 0.66 | 0.33 | 0.38 |
Total Nitrogen (%) | 0.21 | 0.13 | 0.14 |
Organic matter (%) | 1.14 | 0.56 | 0.65 |
C/N ratio | 9.875 | 10.2 | 9.55 |
Total Phosphorous (%) | 0.05 | 0.03 | 0.03 |
Total Potassium (%) | 0.87 | 0.56 | 0.29 |
Isolates | Presence of halo zone | Soluble phosphorous (ppm) |
---|---|---|
Control | - | 0.0977 |
Raoultella ornithinolytica | + | 0.2233 |
Pseudomonas pseudoalcaligenes | - | - |
Bacillus cereus | - | 0.9721 |
Pseudomonas aeruginosa | + | 0.3384 |
Bacillus subtilis | + | 0.2722 |
Name of the organism | ppm P released | |||
---|---|---|---|---|
Period of incubation | ||||
2 days | 4 days | 6 days | 10 days | |
Control | 0.0189 | .0311 | 0.0158 | 0.0005 |
Pseudomonas aeruginosa | 0.0440 | 0.2011 | 0.0518 | 0.0417 |
Bacillus cereus | 0.0576 | 0.1735 | 0.0335 | 0.0293 |
Bacillus subtilis | 0.0665 | 0.1845 | 0.0751 | 0.0466 |
Raoultella ornithinolytica | 0.0717 | 0.1640 | 0.0480 | 0.0311 |
Name of the organism | ppm P released | |||
---|---|---|---|---|
Period of incubation | ||||
2 days | 4 days | 6 days | 10 days | |
Control | 0.0210 | 0.0023 | 0.0316 | 0.0060 |
Pseudomonas aeruginosa | 0.0517 | 0.1666 | 0.0651 | 0.0810 |
Bacillus cereus | 0.0613 | 0.4717 | 0.0786 | 0.0415 |
Bacillus subtilis | 0.0336 | 0.2018 | 0.0718 | 0.0439 |
Raoultella ornithinolytica | 0.0861 | 0.1071 | 0.0499 | 0.0339 |
Name of the organism | ppm P released | |||
---|---|---|---|---|
Period of incubation | ||||
2 days | 4 days | 6 days | 10 days | |
Control | 0.0219 | 0.0285 | 0.0207 | 0.0226 |
Pseudomonas aeruginosa | 0.0732 | 0.0619 | 0.0498 | 0.0514 |
Bacillus cereus | 0.0552 | 0.0534 | 0.0297 | 0.0480 |
Bacillus subtilis | 0.0496 | 0.0483 | 0.0590 | 0.0441 |
Raoultella ornithinolytica | 0.0647 | 0.0504 | 0.0513 | 0.0492 |
At 25˚C and 30˚C, the amount of soluble phosphate in the medium increased on the fourth day of incubation and then decreased gradually. This indicated that organisms showed their highest degradation activity on the fourth day of incubation when incubation temperature was fixed at 25˚C and 30˚C [
Temperature is one of the major environmental factors that influence biodegradation. Furthermore, bacterial metabolism may increase with an increase in temperature (Nadalian, et al., 2016) . In the experiment, temperature was found
to be a significant factor. It is reported that Bacillus species mainly grow in an optimum temperature of nearly 25˚C to 30˚C (Web, 1) , Pseudomonas aeruginosa at 25˚C to 37˚C (Prince, 2012) and Raoutella ornithinolytica at 37˚C (Chung, et al., 2016) . However, by measuring the amount of soluble phosphorous throughout the incubation period, it was demonstrated that for all organisms, 25˚C and 30˚C were the most favorable temperatures for degrading malathion [Figures 8(a)-(d)]. The release of the maximum amount of soluble
phosphate at 25˚C and 30˚C throughout the incubation period indicated the increase in metabolism activity due to increase in growth of bacterial cells. Moreover, Bacillus cereus followed by Pseudomonas aeruginosa was found to be the most efficient malathion degrading bacteria in this experimentby measuring total amount of phosphorous [
It is reported that hydrolysis plays an important role in malathion degradation when pH > 7. This is because malathion is responsive to hydrolysis reaction because of its stronger polar nature. Although malathion may be degraded by chemical processes in soil such as chemical hydrolysis, the amount of microbial degradation is far greater than chemical degradation in natural systems (Mulla, et al., 1981) . Biodegradation also plays a role when pH < 7 and the rate of hydrolysis is slow, relative to the rate of biodegradation (Web, 2) . Different types of enzymes have been reported in organic phosphorous compound degradation. These enzymes can either be acid or alkaline phosphomonoesterases (Jorquera, et al., 2011) . Under this study efficiency of phosphate solubilizing bacteria in degrading malathion has been investigated. Release of inorganic phosphorous implies the practical implication of phosphate solubilizing bacteria in mineralizing malathion.
The authors declare no conflicts of interest regarding the publication of this paper.
Authors would like to thank the Department of Soil, Water and Environment, University of Dhaka and Food Analysis and Research Laboratory, Centre for Advance Research in Sciences, University of Dhaka for the financial support.
Chakma, P., Chowdhury, S., Bari, L., & Hoque, S. (2019). A Feasible Way of Degrading Malathion Pesticide under Laboratory Condition Using Phosphate Solubilizing Bacteria. Journal of Geoscience and Environment Protection, 7, 1-12. https://doi.org/10.4236/gep.2019.71001