TITLE:
Development of Hormone-Active Fiber in the Form of Artificial Insulin Depot
AUTHORS:
A. Medovic Baralic, P. Skundric, Lj. Sretkovic, I. Pajic Lijakovic, M. Kostic
KEYWORDS:
Cation-exchange Fibers; Alginate Fibers; Insulin; Chemisorption; Artificial Depot
JOURNAL NAME:
Engineering,
Vol.4 No.10B,
October
21,
2013
ABSTRACT:
This study examines the
phenomena of the hormone-active fibers obtaining process, in the form of
artificial insulin depot. As a fibrous carrier of insulin cation-exchange
polyacrylonitrile (PAN) fibers and biodegradable polysaccharide alginate fibers
were used. The process of obtaining fibrous artificial insulin depot was based
on the chemisorption of insulin from insulin aqueous solutions by these fibers. The parameters
of insulin chemisorption reaction were determined and their influence on
quantities of bonded insulin in the artificial depot was studied. The impact of
fiber polymer nature on the intensity of insulin chemisorption was studied and
determined. Also, the location and deposition of insulin in and onto the fiber,
fiber topography were studied. The maximum amounts of bounded insulin for the
cation-exchange PAN fibers were 395.0 mg porcine insulin chromatographic / g of
fiber, and for the alginate fibers were about 300 mg of porcine insulin chromatographic / g of
fiber.