TITLE:
Expression of STAT3 in non-small cell lung cancer and its clinical significance
AUTHORS:
Hongcheng Wang, Xiu Lan, Benyan Song, Caixia Xin, Chao Wang, Hong Dai
KEYWORDS:
Signal Transducer and Activator of Transcription 3; Non-Small Cell Lung Cancer; Tumor Marker; ROC Curve; Diagnostic Value
JOURNAL NAME:
Advances in Lung Cancer,
Vol.2 No.2,
June
21,
2013
ABSTRACT: Signal transducer and
activator of transcription 3(STAT3) plays an important role in tumor occurrence
and development. Here we attempt to evaluate the clinical diagnostic value of
STAT3 as a potential tumor marker by detecting the expression of STAT3 in blood
of non-small cell lung cancer (NSCLC) patients. Immunohistochemistry was applied
to detect STAT3 protein in A549 cells and MRC-5 cell, real time PCR was applied
to detect STAT3 mRNA and ELISA was applied to detect the expression of STAT3 protein in NSCLC patients, benign lung disease group and healthy controls. The
expression levels of STAT3 mRNA and STAT3 protein in A549 cells and NSCLC
patients were significantly higher (P P > 0.05). When comparing the Az of
STAT3 protein with CEA, CA125 and CYFRA21-1 respectively, there was no significant difference (P > 0.05). And we
compared the Az of the four joint detection (STAT3 + CEA + CA125 + CYFRA21-1)
with the three joint detection (CEA + CA125 + CYFRA21-1), 0.930 and 0.841, respectively, P