TITLE:
Antimicrobial Activity and Rates of Tannins in Stryphnodendron adstringens Mart. Accessions Collected in the Brazilian Cerrado
AUTHORS:
Giovana Maria Lanchoti Fiori, Ana Lucia Fachin, Valéria S. C. Correa, Bianca Waleria Bertoni, Silvana Giuliatti, Saulo França Amui, Suzelei de Castro França, Ana Maria Soares Pereira
KEYWORDS:
Cerrado; Barbatimão; Genetic Diversity; Phytoterapy
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.11,
November
14,
2013
ABSTRACT: Inner bark extracts of Stryphnodendron
adstringens, a leguminous tree species known as
Barbatimão, are often incorporated to phytotherapic
formulations due to their antimicrobial and healing activities. In this study,
extracts from S. adstringens accessions
collected in 12 distinct locations were investigated in order to determine the
rates of tannins in inner barks and to validate S. adstringens antibacterial
and antifungal effectiveness. Yields of tannins were quantified by colorimetric assay following methodology described in the
Brazilian Pharmacopoeia and the antimicrobial activity was determined by
microdilution technique proposed by the National Committee for Clinical
Laboratory Standards using S. adstringens hydroalcoholic and aqueous extracts
and semi-purified fractions. Investigated extracts did not present significant
antibacterial activity though aqueous extracts exhibited antifungal effect
against both Trichophyton rubrum mutant and clinical strains (MIC 156 μg/mL).
A positive correlation between tannin concentration and antifungal activity
was observed and the accessions collected in Delfinópolis (MG) were considered
elite.