TITLE:
A New SPME Thermal Desorption Interface for HPLC
AUTHORS:
John Jairo Ríos Acevedo, Mohamed Nour Kayali Sayadi, Luis María Polo Díez
KEYWORDS:
SPME-HPLC-FLD; SPME-HPLC Interface; Polynuclear Aromatic Hydrocarbons
JOURNAL NAME:
Journal of Analytical Sciences, Methods and Instrumentation,
Vol.3 No.4,
October
22,
2013
ABSTRACT:
A new SPME/HPLC interface is developed. It is based on thermal desorption from
the SPME fiber and organic solvent collection of the desorbed analytes by
sweeping them with an argon flow into a small organic solvent volume which is
further injected into the HPLC chromatograph. Extraction and desorption
parameters were investigated using five PAHs of different volatilities
(naphthalene, acenaphthalene, fluorene, phenanthrene and anthracene) as test compounds
and fluorimetric detection. Regression coefficients closed to 0.99 with RDS
= 3) and detection limits in the range
0.07 - 0.99 μg.L-1 were found. A method was applied
to determine the above PAHs in water samples. The results were compared with
those supplied by the 550.1 EPA method showing the agreement of both methods at
the 0.05 significance level.