TITLE:
Does Making Method of Alginate Hydrogel Influence the Chondrogenic Differentiation of Human Mesenchymal Stem Cells?
AUTHORS:
Jessica Schiavi, Naceur Charif, Natalia de Isla, Danièle Bensoussan, Jean-François Stoltz, Nadia Benkirane-Jessel, Céline Huselstein
KEYWORDS:
Component: Cartilage Tissue Engineering; Sodium Alginate; Sprayed method; Molded Method
JOURNAL NAME:
Engineering,
Vol.4 No.10B,
October
21,
2013
ABSTRACT:
To overcome cartilage
injury, strategies have been developed in the last few years based on tissue
engineering to rebuild the defects. Cartilage engineering is principally based
on three main biological factors: cells (native cells (chondrocytes) or a more
primitive ones as mesenchymal stem cells), scaffolds and functionalization
factors (growth factors, mechanical stimulation and/or hypoxia). Cartilage
tissue engineering strategies generally result in homogeneous tissue structures
with little resemblance to native zonal organization of articular cartilage.
The main objective of our work concerns the buildup of complex biomaterials
aimed at reconstructing biological tissue with three dimensional cells
construction for mimicking cartilage architecture. Our strategy is based on
structures formation by simple and progressive spraying of mixed alginate
hydrogel and human mesenchymal stem cells (hMSC). In this work, the comportment
of cells and more precisely their chondrogenic differentiation potential is
compared to a traditional making process: the mold. We report here that spraying method allowed to product
a scaffold with hMSC that confer a favorable environment for neocartilage
construction.