TITLE:
Genetic diversity of hybrid durian resulted from cross breeding between Durio kutejensis and Durio zibethinus based on random amplified polymorphic DNAs (RAPDs)
AUTHORS:
Tati Hariyati, Joni Kusnadi, Estri Laras Arumingtyas
KEYWORDS:
RAPD; Durio kutejensis; Durio zibethinus; Genetic Diversity
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.3 No.3,
July
17,
2013
ABSTRACT:
One of the ways to improve
the quality of Indonesian Durian is by utilizing germplasm diversity. Durio zibethinus is the most cultivated durian in Indonesia, whereas Durio kutejensis is a unique durian cultivar which has golden yellow
fruit flesh without smell. Crossbreeding of those two cultivars, in order to generate superior Durian cultivars has been done. Genetic diversity of durian
generates from cross breeding between D. kutejensis and D. zibethinus was identified in molecular level using RAPD technique.
Among 20 primers used in this study, 5 primers: OPA-02, OPA-03, OPA-08,
OPA-10 and OPA-13 were capable of differentiating both the parents and the
hybrids. RAPD analysis resulted in genetic diversity of hybrid Durian with
family relationship of 0.59%-0.1%. Hybrids UB1, UB5, UB13, UB19, UB21, UB7
and UB35 have similarity value of 0.81% with their parent DRCK, whereas hybrids
UB8, UB10, UB18 and UB17 have similarity value of 0.70% with their parent
DRCM1. Hybrids UB2, UB16 and UB22 belong to one group with similarity value of
0.67%. Three hybrids lines UB2, UB16 and UB22 show the highest distance to both
parent. The rest of the hybrids lines grouped into similar cluster to the
parents D. kutejensis, whereas the
other parent (D. zibethinus) belong
to different cluster separated from all other hybrid lines and parents.